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We narrowed to 16,087 results for: grna

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Showing: 521 - 540 of 16087 results

  4. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

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  18. pUC57kan-T7-gRNA-U6 V2

    Plasmid
    #115520
    Purpose
    To construct multiple sgRNA expression vector for CRISPR
    Depositor
    Xingxu Huang
    Insert
    sgRNA scaffolf-U6 promoter
    Use
    CRISPR
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    115520-plasmid-map-sequence-id-224556

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  20. pU6_B2M_sgRNA(PP7)

    Plasmid
    #232438
    Purpose
    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop
    Depositor
    Gil Westmeyer
    Article
    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.
    Insert
    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    Use
    CRISPR
    Promoter
    U6
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    232438-plasmid-map-sequence-id-462755

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Showing: 521 - 540 of 16087 results