We narrowed to 1,601 results for: tef

1. pCfB2795

   Plasmid

   #63650

   Purpose

   pCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct)

   Plasmid

   #213402

   Purpose

   douple floxed, myristoylation (myr) site for membrane targeting and EGFP

   Depositor

   Katrin Willig

   Article

   Wegner et al Elife. 2022 Feb 23;11:e73603. doi: 10.75

   Insert

   EGFP
   

   Use

   AAV

   Tags

   C-terminal (Ct) cytoplasmic domains of low densit…

   Expression

   Mammalian

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FEMP

   Plasmid

   #191973

   Purpose

   FRET-based indicator of ER-mitochondria proximity (FEMP)

   Depositor

   Luca Scorrano

   Article

   Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

   Insert

   AkapYFP-Tav2a-CFP-sac1
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE-FLEX-eNpHR-EYFP

   Plasmid

   #89876

   Purpose

   To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   eNpHR=EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. SZ_EC_02

   Plasmid

   #155341

   Purpose

   Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR

   Depositor

   Jens Nielsen

   Article

   Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:

   Inserts

   tef1 promoter - xylulose kinase - pex20 terminator
   pyk1 promoter - xylose reductase - pex16 terminator
   gapdh promoter - xylitol dehydrogenase - lip2 terminator
   

   Use

   Easycloneyali insert vector

   Expression

   Yeast

   Promoter

   gapdh, pyk1, and tef1

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-ZFN-hSyn-DIO-PSD95-FingR-Citrine-CCR5TC

   Plasmid

   #213398

   Purpose

   transcriptionally regulated, double floxed, postsynaptic marker of PSD95 fused to Citrine

   Depositor

   Katrin Willig

   Article

   Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

   Insert

   FingR for endogenous PSD95, transcriptinally regulated
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-mCherry-CyrCR1

   Plasmid

   #128768

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICH47822_HA-Sr62TK

   Plasmid

   #233535

   Purpose

   Expresses HA-Sr62TK in planta

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   Sr62TK
   

   Tags

   HA tag

   Expression

   Plant

   Promoter

   35S

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH47822_Sr62NLRa

   Plasmid

   #233537

   Purpose

   Expresses Sr62NLRa in planta

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   Sr62NLRa
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCfB2793

    Plasmid

    #64043

    Purpose

    pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    TEF1 promoter and none

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAS9c

    Plasmid

    #141250

    Purpose

    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAS9cd

    Plasmid

    #141252

    Purpose

    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

    Plasmid

    #128755

    Purpose

    Expresses CYRANO gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    CYRANO sgRNA (OIP5-AS1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB2798

    Plasmid

    #63659

    Purpose

    pCfB2798 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    TEF1 promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid

    #202571

    Purpose

    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    LINE-1
    

    Tags

    GFP-AI retrotransposition reporter and HaloTag7 o…

    Expression

    Mammalian

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-mCherry-CyrCR1.miR16

    Plasmid

    #128747

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETM11-6xHis-TEV-hORF1p_K3AK4A

    Plasmid

    #202585

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Lysine 3 to Alanine, changed ORF1p …

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCfB2794

    Plasmid

    #63651

    Purpose

    pCfB2794 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    TEF1 promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-FOXO3a WT-ER

    Plasmid

    #8355

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    mouse estrogen receptor (G525R) on C-term

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-FOXO3a WT delta CT

    Plasmid

    #1796

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CT (aa468-673)

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only