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pRetroX SgK269 WT

Plasmid

#68028

Purpose

expression of Sgk269 in mammalian cells

Depositor

Roger Daly

Article

Croucher et al Cancer Res. 2013 Mar 15;73(6):1969-80. d

Insert

PEAK1 (PEAK1 Human)

Use

Retroviral

Tags

FLAG/myc, HA, and IRES-DsRed

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pTag-RFP-C-h-Rab4a-c-Myc

Plasmid

#79800

Purpose

Express TagRFP labeled human Rab5a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab4a (RAB4A Human)

Use

Tags

TagRFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pMXs3-NRAS G13D

Plasmid

#86144

Purpose

Retroviral vector expressing NRAS(G13D)-IRES-RFP

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

NRAS G13D (NRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

G13D

Promoter

LTR

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pMX-IY HA-GBRPL2

Plasmid

#89298

Purpose

For expression of the mammalian Atg8 protein GABARAPL2 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Retroviral

Tags

HA and IRES-YFP

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMX-IY HA-GBRPL1

Plasmid

#89297

Purpose

For expression of the mammalian Atg8 protein GABARAPL1 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL1 (GABARAPL1 Human)

Use

Retroviral

Tags

HA and IRES-YFP

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-HA-C1orf27

Plasmid

#86122

Purpose

Retroviral vector expressing HA-C1orf27-IRES-Bsd

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

C1orf27 (C1orf27 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBEL1200

Plasmid

#138526

Purpose

For expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.

Depositor

Gira Bhabha, Damian Ekiert

Article

Ekiert et al Cell. 2017 Apr 6;169(2):273-285.e17. doi

Insert

mlaFEDCB (mlaF, mlaE, mlaD, mlaC, mlaB E. coli)

Use

Tags

N-terminal His tag and TEV cleavage site on MlaD

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMX-IY LC3A

Plasmid

#89290

Purpose

For expression of the mammalian Atg8 protein LC3A without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

LC3A (MAP1LC3A Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-230

Plasmid

#90499

Purpose

pSIN - EF-1alpha - PIF3 - MTAD - IRES - PhyB - GBD, dual vector of PIF3-MTAD under EF-1 alpha promoter and PhyB-DBD under IRES promoter; See growth conditions below.

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-tFD and mito-tFNR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-StefinA

Plasmid

#182419

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-stefin (CSTA Human)

Use

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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