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Plasmid#216515PurposeExpresses MBP-tagged full length hnRNPAB X2DepositorInserthnRNPAB X2 FL
UseTags6xHis tags-MBPExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-dgat1a in situ probe
Plasmid#232137Purposezebrafish dgat1a anti-sense in situ probe, linearize with NotI, SP6 polymeraseDepositorInsertdgat1a (dgat1a Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-mogat2 in situ probe
Plasmid#232139Purposezebrafish mogat2 anti-sense in situ probe, linearize with NotI, SP6 polymeraseDepositorInsertmogat2 (mogat2 Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
TAK8
Plasmid#227096PurposeLuciferase reporter vector with wild type 5' Element (MMTV) insertDepositorInsertCMV Promoter and R to 400bp Gag
UseLuciferaseTagsExpressionMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
hnRNPAB X2-IDR
Plasmid#216517PurposeExpresses MBP-tagged intrinsically disordered region of hnRNPAB X2DepositorInserthnRNPAB X2-IDR
UseTags6xHis tags-MBPExpressionBacterialMutationdeleted amino acids 2-225PromoterAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-memRFP-3xPax7gRNA
Plasmid#224569PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.DepositorInsertmRFP1
UseCRISPRTagsMembrane localization signalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xPax7gRNA
Plasmid#224570PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xGFP11-mem-3xPax7gRNA
Plasmid#224571PurposeUbiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.DepositorInsert3xGFP11
UseCRISPRTagsMembrane Localization SignalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
JD226
Plasmid#229484PurposeAdenoviral helper plasmid containing E2A, VA RNA I and L4-22k genesDepositorInsertE2A, VA RNA I, L4-22k
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterCMV (E2A), Ef1a (L4-22k) and wt promoter (VA RNA …Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-TSen
Plasmid#209916PurposeA FRET biosensor for ADAM17 activityDepositorInsertTSen
UseTagsECFP and YPetExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
TurboID-MEK1
Plasmid#226169PurposeUsed to transiently express a TurboID fusion of the protein kinase MEK1.DepositorInsertMyc-TurboID-MEK1 (MAP2K1 Human)
UseTagsMyc-TurboIDExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-PARP7
Plasmid#184474PurposeFor gateway cloning of PARP7DepositorInsertPARP7 (TIPARP Human)
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-PARP15
Plasmid#184471PurposeFor gateway cloning of PARP15DepositorInsertPARP15 (PARP15 Human)
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
RcSxph
Plasmid#194018PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
UseTagsHis10 and mEGFP,ExpressionMutationPromoterPolyhedrinAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
Plasmid#222990PurposeBacterial expression of iGeoCas9(C1) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
p931-SWITCH-OVER insert: EF1a-Blasti
Plasmid#217890Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p223-Pulse-Switch
Plasmid#217889PurposeLentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P
Plasmid#216404PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51, 56, Glutamine 70, 443 and Asp…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-FL-syt7
Plasmid#213630PurposeBacterial expression of SUMO-tagged mouse FL-syt7DepositorInsertFL-syt7 (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN0617 (pDEST-mEGFP C-term, NatR)
Plasmid#210484PurposepDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsmEGFPExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only