Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 29,763 results for: REP

Showing: 8071 - 8100 of 29763 results

  1. kiCAP-AAV-BI30

    Plasmid
    #183749
    Purpose
    AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.
    Depositor
    Ben Deverman
    Article
    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40
    Insert
    AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
    Use
    AAV
    Tags
    Expression
    Mutation
    K449R, and NNSTRGG inserted between 588 and 589 o…
    Promoter
    p41
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    183749-plasmid-map-sequence-id-442496

    Enlarge

  5. pAR-9.47_VP2_AS

    Plasmid
    #74241
    Purpose
    Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.
    Depositor
    Miguel Sena-Esteves
    Article
    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2
    Inserts
    AAV2 Rep
    AAV9.47-VP2_AS
    Use
    AAV
    Tags
    19 alanines fused to VP2 of AAV9.47
    Expression
    Mutation
    Promoter
    Available Since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74241-plasmid-map-sequence-id-141992

    Enlarge

  6. FLAG-D1-IRES-SS-D2-IRES-HA-D3

    Plasmid
    #215119
    Purpose
    Expresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian cells from an inducible lentiviral vector.
    Depositor
    Michele Pagano
    Article
    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012
    Inserts
    CCND1 (CCND1 Human)
    CCND2 (CCND2 Human)
    CCND3 (CCND3 Human)
    Use
    Lentiviral
    Tags
    FLAG-tag, HA-tag, and SS (STREP-STREP)
    Expression
    Mammalian
    Mutation
    Promoter
    IRES and TREG3
    Available Since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    215119-plasmid-map-sequence-id-426788

    Enlarge

  7. pRRLSIN.cPPTLuciferase.WPRE

    Plasmid
    #69251
    Purpose
    luciferase reporter in lentivirus backbone
    Depositor
    Stephen Tapscott
    Insert
    Luciferase
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    none
    Available Since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    69251-plasmid-map-sequence-id-122039

    Enlarge

  8. pGL4.11 Nued2

    Plasmid
    #59744
    Purpose
    Luciferase reporter plasmid for assessing neuronal activity-regulated enhancer function
    Depositor
    Michael Greenberg
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    59744-plasmid-map-sequence-id-93965

    Enlarge

  9. pVI

    Plasmid
    #31597
    Depositor
    Michele Calos
    Insert
    phiC31 integrase (int Streptomyces phage)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 18, 2011
    Availability
    Academic Institutions and Nonprofits only
    31597-plasmid-map-sequence-id-14491

    Enlarge

  10. pRRLSIN.cPPT.RFPL4b.Luciferase.WPRE

    Plasmid
    #69252
    Purpose
    Luciferase in lentivirus backbone to report Dux4 activation of RFPL4b promoter
    Depositor
    Stephen Tapscott
    Insert
    RFPL4b (RFPL4B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    RFPL4b
    Available Since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    69252-plasmid-map-sequence-id-123983

    Enlarge

  11. pGL3 RFPL4b promoter

    Plasmid
    #69250
    Purpose
    Luciferase reporter for Dux4 activation of RFPL4b transcription
    Depositor
    Stephen Tapscott
    Insert
    RFPL4b (RFPL4B Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    RFPL4b
    Available Since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pGBScomp High

    Plasmid
    #223202
    Purpose
    Group B Streptococcus High Expression Modular Complementation Vector
    Depositor
    Thomas Hooven
    Insert
    N/A
    Use
    Complementation vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    223202-plasmid-map-sequence-id-451880

    Enlarge

  13. pGBScomp Low

    Plasmid
    #223203
    Purpose
    Group B Streptococcus Low Expression Modular Complementation Vector
    Depositor
    Thomas Hooven
    Insert
    N/A
    Use
    Complementation vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    223203-plasmid-map-sequence-id-451879

    Enlarge

  14. pDCyR6

    Plasmid
    #172558
    Purpose
    Inducible gene expression, pCDF origin, CymRAM repressor, PCymRC promoter, mRFP, Streptomycin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    CymRAM-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PCymRC
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172558-plasmid-map-sequence-id-346957

    Enlarge

  15. pELlR6

    Plasmid
    #172562
    Purpose
    Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Streptomycin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LacI-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLlacO-1
    Available Since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    172562-plasmid-map-sequence-id-347238

    Enlarge

  16. pDLlR6

    Plasmid
    #172559
    Purpose
    Inducible gene expression, pCDF origin, LacI repressor, PLlacO-1 promoter, mRFP, Streptomycin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LacI-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLlacO-1
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172559-plasmid-map-sequence-id-346958

    Enlarge

  17. pGBSedit Sham

    Plasmid
    #223200
    Purpose
    Group B Streptococcus Efficient Genome Editing with Modular crRNA
    Depositor
    Thomas Hooven
    Insert
    N/A
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    223200-plasmid-map-sequence-id-451877

    Enlarge

  18. pX330-Flag-eSpCas9-D10A

    Plasmid
    #80449
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_D10A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available Since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80449-plasmid-map-sequence-id-157945

    Enlarge

  19. pX330-Flag-eSpCas9-H840A

    Plasmid
    #80454
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available Since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80454-plasmid-map-sequence-id-151472

    Enlarge

  20. pJK275

    Plasmid
    #73363
    Purpose
    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)
    Depositor
    T. J. Kappock
    Insert
    delta-8 sphingolipid desaturase
    Use
    Tags
    Expression
    Yeast
    Mutation
    missing residues 1-6
    Promoter
    GAL1
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73363-plasmid-map-sequence-id-138870

    Enlarge

  21. pJK616

    Plasmid
    #72460
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with an N-terminal His6 tag (H6TdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72460-plasmid-map-sequence-id-133770

    Enlarge

  22. pBIR-GFP

    Plasmid
    #49807
    Purpose
    In vivo GFP based system to measure break induced replication (BIR) repair efficiency. expression of I-SceI generates a double strand break that when repaired by BIR mechanism will restore GFP.
    Depositor
    Thanos Halazonetis
    Insert
    GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    beta-actin
    Available Since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    49807-plasmid-map-sequence-id-74303

    Enlarge

  24. pENTR ARRET

    Plasmid
    #111384
    Purpose
    ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
    Depositor
    Klaus Holzmann
    Insert
    ARRET
    Use
    Gateway entry cloning
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    111384-plasmid-map-sequence-id-209223

    Enlarge

  26. C64m

    Plasmid
    #120980
    Purpose
    MoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).
    Depositor
    Richard Murray
    Insert
    temperature sensitive lambda repressor (cI857)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120980-plasmid-map-sequence-id-236034

    Enlarge

  27. pGL3_RNF157_wt

    Plasmid
    #232381
    Purpose
    RNF157 enhancer, wt without one repeat re-synthesized
    Depositor
    Joanna Wysocka
    Insert
    RNF157 (RNF157 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    RNF157 enhancer, wt without one repeat re-synthes…
    Promoter
    Available Since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232381-plasmid-map-sequence-id-461192

    Enlarge

  28. pX330-Flag-HFSpCas9-H840A

    Plasmid
    #80455
    Purpose
    high fidelity SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80455-plasmid-map-sequence-id-151476

    Enlarge

  29. pGBScrispri Sham

    Plasmid
    #223201
    Purpose
    Group B Streptococcus inducible dCas12 CRISPRi with Modular crRNA
    Depositor
    Thomas Hooven
    Insert
    N/A
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    223201-plasmid-map-sequence-id-451878

    Enlarge

Showing: 8071 - 8100 of 29763 results