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pBSKS Notch4

Plasmid

#21325

Depositor

Jan Kitajewski

Article

Uyttendaele et al Development. 1996 Jul . 122(7):2251-9.

Insert

Notch4 (Notch4 Mouse)

Use

Cloning vector

Available Since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only
Anti-MFF [N382/14]

Plasmid

#190318

Purpose

Mammalian Expression Plasmid of anti-MFF (Human). Derived from hybridoma N382/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-MFF (Homo sapiens) recombinant Mouse monoclonal antibody (MFF Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B BimEL (3SA)

Plasmid

#23091

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (3SA) (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 55/65/73 to alanine

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pOP459

Plasmid

#85933

Purpose

Encodes the Anti-Ebola 2G4 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

Depositor

Timothy Lu

Article

Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

Insert

Anti-Ebola 2G4 monoclonal antibody

Expression

Yeast

Promoter

AOX1

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pHA-ΔN335-Kin1A

Plasmid

#31606

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN335 kinesin-1A (Kif5a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 1-335

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
Venus Cam KII alpha mutant T286D

Plasmid

#29429

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Tags

Venus

Expression

Mammalian

Mutation

T286D mutation in CamKIIa sequence

Available Since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
ManII-SBP-EGFP_puromycin

Plasmid

#65260

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSuper-Sema4A 42-3n

Plasmid

#115173

Purpose

shRNA against rodent Sema4A

Depositor

Suzanne Paradis

Article

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

shRNA targeting Sema4A

Use

RNAi

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTR AE Clover

Plasmid

#90240

Purpose

Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (AE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Promoter

PGK

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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LightR-bRaf-mVenus

Plasmid

#162154

Purpose

Expresses LightR-bRaf-mVenus in mammalian cells

Depositor

Andrei Karginov

Article

Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

Insert

LightR-bRaf-mVenus

Tags

mVenus

Expression

Mammalian

Mutation

Valine 600 is substituted with Glutamic acid

Promoter

CMV

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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Cntn1-AP-His

Plasmid

#71939

Purpose

Expresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn1 (Cntn1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK JunD-HA neo

Plasmid

#58515

Purpose

Lentiviral expression vector for an inducible HA-tagged mouse JunD

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JunD (Jund Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-mSNX27-ΔF3

Plasmid

#163618

Purpose

mouse SNX27 without the F3 domain cDNA C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-ΔF3 (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

deletion F3 domain

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCS-TRE-FHDnd1-Ubc-rtTA-I2G

Plasmid

#70076

Purpose

2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Silent mutations are introduced to escape from RN…

Promoter

Tet responsible promoter

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-Talin-N-10

Plasmid

#55139

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Paszek et al Nat Methods. 2012 Jul 1;9(8):825-7. doi:

Insert

Talin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pLVXT-mTcf3

Plasmid

#121935

Purpose

doxycycline-inducible mouse Tcf3

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Transcription Factor 3 (Tcf3 Mouse)

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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FFTTH--GFP-Amotl2

Plasmid

#112833

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Insert

Amotl2 (Amotl2 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Ngn3 Cre (DM#259)

Plasmid

#15023

Depositor

Douglas Melton

Article

Gu et al Development. 2002 May . 129(10):2447-57.

Insert

Ngn3 promoter (Neurog3 Mouse)

Use

Cre/Lox; Mouse transgenic

Tags

Cre

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
Sema4d-Fc-His

Plasmid

#72157

Purpose

Expresses the extracellular region of the Sema4D protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4d (Sema4d Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-Arl13b1

Plasmid

#40870

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Gateway entry

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only