We narrowed to 8,984 results for: FIE

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pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

Plasmid

#167832

Purpose

Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

HRAS(N17) (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S17N

Promoter

Ubc, TRE

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO1

Plasmid

#52258

Purpose

Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-1 (SUMO1 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 )
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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Nsp3 Mac1 - C2 (UCSF)

Plasmid

#169209

Purpose

Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

Depositor

James S Fraser

Article

Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

Insert

SARS-CoV-2 Nsp3 Mac1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-N MCV ER

Plasmid

#37860

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

MCV ER (MCPyV_gp2, MCPyV_gp1 Human Merkel Cell Virus)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pUb FLAG-tws

Plasmid

#195068

Purpose

Expresses FLAG-tagged Drosophila tws protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

twins (tws Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUAP1

Plasmid

#63674

Purpose

Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.

Depositor

Nicola Patron

Article

Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np

Insert

RFP cloning selection cassette

Use

Synthetic Biology

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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Nsp3 Mac1 - P43 (Oxford)

Plasmid

#169210

Purpose

Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

Depositor

James S Fraser

Article

Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

Insert

SARS-CoV-2 Nsp3 Mac1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSrTA1662-toolkit

Plasmid

#154372

Purpose

Expresses SrTA1662 in plants

Depositor

Brande Wulff

Article

Gaurav et al Nat Biotechnol. 2022 Mar;40(3):422-431.

Insert

SrTA1662

Expression

Bacterial and Plant

Promoter

Native promoter

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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AiP14945 - pAAV-AiE0410m-minBG-iCre(R297T)-3XmiR126TS-BGHpA

Plasmid

#254824

Purpose

Encodes an AAV that expresses Cre specifically in oligodendrocytes

Depositor

Allen Institute for Brain Science, Boaz Levi, Jonathan Ting

Article

Mich et al eLife 14:RP108640

Insert

iCre

Use

AAV

Mutation

R297T for less recombinase activity which yields …

Available Since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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FGFR2 3xFlag

Plasmid

#110063

Purpose

FGFR2 expression in mammalian cells (JCOPCO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR2 F276C 3xFlag

Plasmid

#110064

Purpose

FGFR2 F276C expression in mammalian cells (JCOPO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

mutated Phenylalanine 276 to Cysteine (F276C)

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR2 K41E 3xFlag

Plasmid

#110105

Purpose

expresses FGFR2 with a single amino acid change in mammalian cells

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

mutated Lysine 41 to Glutamic acid (K41E)

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_18GZG

Plasmid

#117228

Purpose

shble flanked by an endogenous GAPDH promoter and terminator system

Depositor

Jackie Collier

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

shble

Use

Thraustochytrid expression

Promoter

GAPDH

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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DinoIII-neo

Plasmid

#155026

Purpose

Dinoflagelate backbone for amino 3'-glycosyl phosphotransferase (neo) gene

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

amino 3'-glycosyl phosphotransferase

Expression

Bacterial

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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DinoIII-pat

Plasmid

#155028

Purpose

Dinoflagelate backbone for Phosphinothricin N-acetyltransferase, pat, gene

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

Phosphinothricin N-acetyltransferase

Expression

Bacterial

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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DinoIII-bsr

Plasmid

#155027

Purpose

Dinoflagelate backbone for blasticidin S-resistance gene (bsr)

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

Blasticidin-S deaminase

Expression

Bacterial

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pUC:FCP:ShBle:FCP:EGFP

Plasmid

#85987

Purpose

Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

Depositor

Thomas Mock

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Inserts

ShBle
EGFP

Promoter

FCP

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pLBS.CAG

Plasmid

#129349

Purpose

Lentivirus vector

Depositor

Yasushi Okada

Insert

unspecified

Use

Lentiviral

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCS2P+

Plasmid

#17095

Depositor

Marc Kirschner

Type

Empty backbone

Use

Xenopus/mammalian/avian/zebrafish

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pJK317

Plasmid

#72472

Purpose

puc118 modified to contain ribosome binding site

Depositor

T. J. Kappock

Type

Empty backbone

Expression

Bacterial

Promoter

lac

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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