We narrowed to 6,088 results for: pcDNA 3.1

1. L2376-FKBP-GPA-20-114

   Plasmid

   #222896

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA-20GS-NanoLuc11S
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. L2374-FRB-GPA-20-11S

   Plasmid

   #222895

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc11S
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. L2373-FRB-GPA-20-114

   Plasmid

   #222894

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc114
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L2179-FRB-GPA-6-GFPS

   Plasmid

   #222893

   Purpose

   Biosensor chain detecting rapamycin and responding with split GFP reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 6 GS linker, split GFP fragment small.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-6GS-GFPS
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. L2110-FKBP-GPA-20-114

   Plasmid

   #222881

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA-20GS-NanoLuc114
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. L2108-FRB-GPA-20-114

   Plasmid

   #222879

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc114
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. L2109-FRB-GPA-20-11S

   Plasmid

   #222880

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc11S
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. eGFP-SUN1ΔN

   Plasmid

   #213508

   Purpose

   Expresses human eGFP-SUNΔN in mammalian cells

   Depositor

   Karim Mekhail

   Article

   Shokrollahi et al bioRxiv 2023.05.07.539750

   Insert

   eGFP-SUNΔN
   

   Expression

   Mammalian

   Mutation

   SUN1ΔN has the first 138 amino acid (lamina domai…

   Promoter

   CMV

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NSP2-Flag

   Plasmid

   #210344

   Purpose

   Expresses SARS-CoV-2 NSP2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP2 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TLNRD1_35-345_eGFP

    Plasmid

    #208881

    Purpose

    Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    TLNRD1 (TLNRD1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acid sequence 35-345

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TLNRD1_5H_eGFP

    Plasmid

    #208861

    Purpose

    Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    TLNRD1 5 helix bundle (TLNRD1 Human)
    

    Expression

    Mammalian

    Mutation

    Deleted amino acid 142-273

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. SEC61B-2Strep

    Plasmid

    #210375

    Purpose

    Expresses SEC61B fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SEC61B (SEC61B Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TMEM106B-2Strep

    Plasmid

    #210360

    Purpose

    Expresses TMEM106B fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    TMEM106B (TMEM106B Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. ALIX(P801G)-mNeonGreen

    Plasmid

    #191192

    Purpose

    Full-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (P801G) (PDCD6IP Synthetic, Human)
    

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Mutation

    Pro 801 mutated to Gly

    Promoter

    CMV

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

    Plasmid

    #169597

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9 - VP64 fusion
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVH19-Tier1-PhCMV-p300core

    Plasmid

    #169572

    Purpose

    Tier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P300core (EP300 Human)
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS2304-Tier1-Tactb-PPGK-NLuc_TB

    Plasmid

    #169549

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTS1046-Tier1-PhCMV-4xCox8-mRuby2

    Plasmid

    #169537

    Purpose

    Tier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTS2380-Tier1-PhCMV-TM

    Plasmid

    #169485

    Purpose

    Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) (PhCMV-TM-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Junctophilin 2 transmembrane domain (JPH2 Human)
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGlomyc-Ovol1

    Plasmid

    #172871

    Purpose

    Mammalian expression vector with myc-tagged mouse Ovol1 coding sequence

    Depositor

    Renee van Amerongen

    Article

    Mourao et al bioRxiv 2021.06.22.449461

    Insert

    Ovol1 (Ovol1 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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