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pHR-UCOE-SFFV-Zim3-dCas9-BFP

Plasmid

#188776

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv

Plasmid

#217437

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-oA ONE-GO

Plasmid

#189732

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-oA ONE-GO
GNAO (Gnao1 Rat)

Use

Lentiviral

Mutation

I250 (silent mutation in YFP)

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pPB-EF1A-Puro-hTBK1-EGFP

Plasmid

#221553

Purpose

Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

TBK1 (TBK1 Human)

Use

Piggybac

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPEgRNA

Plasmid

#172716

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

gRNA

Expression

Bacterial

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHCMV_DENV4_PrME

Plasmid

#207266

Purpose

Mammalian expression of Dengue virus 4 PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DENV4_PrME (POLY Dengue virus 4)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

Plasmid

#247160

Purpose

Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Puromycin resistance, mCherry

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-PGK-eGFP

Plasmid

#193023

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (PGK) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCSF107mT-GATEWAY-3'-Myc tag

Plasmid

#67617

Purpose

A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-terminal Myc

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP

Plasmid

#162823

Purpose

Doxycycline-inducible overexpression of eGFP

Depositor

Matthew Steinhauser

Article

Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Mutation

V163A

Promoter

Tet ON

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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