We narrowed to 59,446 results for: cloning

1. pUC57-Kan_FR13_APikL2

   Plasmid

   #123648

   Purpose

   Level 0 Golden-Gate compatible vector

   Depositor

   Sophien Kamoun

   Article

   Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

   Insert

   FR13_APikL2
   

   Use

   Golden gate

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pICSL50006

   Plasmid

   #50319

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, luciferase (Photinus pyralis)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pICSL30005

   Plasmid

   #50299

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   N terminal FLAG tag (3x FLAG octapeptide)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR-L5-oPRE-L2

   Plasmid

   #32414

   Depositor

   Matthew Nolan

   Article

   White et al Front Mol Neurosci. 2011;4:8. Epub 2011

   Insert

   oPRE
   

   Use

   Gateway cloning vector

   Mutation

   WPRE with deletion of X protein promoter and muta…

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pICH45152

   Plasmid

   #50263

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter, cab1 (AT1g29930, A. thaliana)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pICH71421

   Plasmid

   #51834

   Purpose

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   plant 3'UTRpolyadenylation signal/terminator, Tom H4
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pICH42211

   Plasmid

   #50255

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter nos (A. tumefaciens)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MXS_CMV::tTA2-bGHpA

   Plasmid

   #62447

   Purpose

   MXS_chaining vector with CMV::tTA2-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   cassette containing the tetracycline transactivator with CMV promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GGE294

   Plasmid

   #120760

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 3 (P3_4UASxpr-TEF)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   P3_4UASxpr-TEF
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCZGY2844

    Plasmid

    #87760

    Purpose

    Punc-17b-PH::miniSOG, expression of membrane targeted PH-miniSOG in cholinergic motor neurons.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Punc-17b-PH-miniSOG
    

    Tags

    PH

    Expression

    Worm

    Promoter

    Punc-17b

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCZGY2162

    Plasmid

    #110136

    Purpose

    Epidermal Mitochondrial-tagged Florescent Calcium Sensor

    Depositor

    Andrew Chisholm

    Article

    Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

    Insert

    mito::GCaMP3
    

    Tags

    mito::GCaMP3

    Expression

    Worm

    Promoter

    Pcol-19

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICSL30004

    Plasmid

    #50302

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS no stop codon, YFP variant of GFP (Aequorea victoria)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Mouse NCKX6 (pcDNA3.1+)

    Plasmid

    #75215

    Purpose

    Mammalian expression of SLC8B1

    Depositor

    Jonathan Lytton

    Article

    Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

    Insert

    SLC8B1 (Slc8b1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Mouse NCKX4-AS-long (pcDNA3.1+)

    Plasmid

    #75208

    Purpose

    Mammalian expression of SLC24A4

    Depositor

    Jonathan Lytton

    Article

    Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

    Insert

    SLC24A4 (Slc24a4 Mouse)
    

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pICSL50011

    Plasmid

    #50317

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, EOS photoactivatable fluorescent protein (L. hemprichii)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p663-UBC-miniTurbo-V5-CDK16_IDG-K

    Plasmid

    #158173

    Purpose

    Gateway destination clone of CDK16 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK16 (CDK16 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICSL80001

    Plasmid

    #50326

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, luciferase (P. pyralis)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pICSL80004

    Plasmid

    #50325

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p667-UBC-DYRK2-FullLength-V5-miniTurbo_IDG-K

    Plasmid

    #198010

    Purpose

    Gateway destination clone of DYRK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    DYRK2-V5-miniTurbo (DYRK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GGE252

    Plasmid

    #120791

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsDown_GSY-NotI
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge