We narrowed to 6,132 results for: pCas
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Plasmid#145094PurposeExpressing base editor nCDA1-NGBE3 in yeast cellsDepositorInsertnCDA1-NGBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-MYO1C
Plasmid#227305PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.DepositorInsertsgRNA Targeting N-terminus of MYO1C (MYO1C Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgRNA2-RPB1-N-term
Plasmid#195111PurposeVector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).DepositorInsertsgRNA against first exon of RPB1 (N-terminal)
UseCRISPRExpressionMammalianAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-VA-NG
Plasmid#220896PurposeExpress haA3A-CBE-VA-NG (with SpCas9-NG variant) in mammalian cellsDepositorInserthaA3A-CBE-VA-NG
ExpressionMammalianAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAEL007097-Cas9
Plasmid#100705PurposeThe promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL007097-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL007097 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT25_GalL_BE3
Plasmid#143736PurposeExpresses BE3 in yeast cellsDepositorInsertBE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHeCAHS-mEGFP-NLS
Plasmid#205014PurposeThe vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the chas gene from H. exemplaris
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJT82_GalL_nCDA1Δ198-BE3
Plasmid#145046PurposeExpresses nCDA1Δ198-BE3 in yeast cellsDepositorInsertnCDA1Δ198-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-198AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgRNA2-2-RPB1-C-term
Plasmid#195109PurposeVector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).DepositorInsertsgRNA against last exon of RPB1 (C-terminal)
UseCRISPRExpressionMammalianAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAEL003877-Cas9
Plasmid#100581PurposeThe promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL003877-hspcas9-GFP
Opie-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL003877 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-vCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10
Plasmid#197979PurposeT-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.DepositorInsertspco-vCasphi-2
AtPDS3 gRNA10
UseCRISPRExpressionPlantPromoterpUBQ10Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
VQR variant
Plasmid#138560PurposeExpresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSDDepositorInsertVQR
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, R1335Q, T1337RPromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgRNA3-RPB1-N-term
Plasmid#195112PurposeVector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).DepositorInsertsgRNA against first exon of RPB1 (N-terminal)
UseCRISPRExpressionMammalianAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgRNA1-RPB1-N-term
Plasmid#195110PurposeVector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).DepositorInsertsgRNA against first exon of RPB1 (N-terminal)
UseCRISPRExpressionMammalianAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
CP1028-ABE8e-dimer
Plasmid#138498PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)
ExpressionMammalianAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pR008_APAD
Plasmid#204818PurposeExpression Apobec1-ADAR2dd fusion protein as PIE-RBP controlDepositorInsertrat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)
TagsHA and Flag and huADAR2ddExpressionMammalianPromoterchicken β-actin promoterAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mRFP1 gRNA
Plasmid#117052PurposeIn vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoterDepositorInsertanti-mRFP1 SpCas9 gRNA
ExpressionBacterialPromoterT7Available SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10
Plasmid#197966PurposeT-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.DepositorInsertspco-vCasphi-2
AtPDS3 gRNA10
UseCRISPRExpressionPlantPromoterCmYLCVp and pUBQ10Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgRNA2-CTCF-prom
Plasmid#195104PurposeVector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).DepositorInsertsgRNA against CTCF promoter
UseCRISPRExpressionMammalianAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgRNA1-CTCF-prom
Plasmid#195103PurposeVector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).DepositorInsertsgRNA against CTCF promoter
UseCRISPRExpressionMammalianAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only