We narrowed to 10,056 results for: transfer

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pAAV-VTKD5

Plasmid

#170850

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of any transgene in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6

Plasmid

#170851

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CNTF-HA

Plasmid

#195517

Purpose

Expresses HA-tagged CNTF in mammalian cells

Depositor

Kevin Park

Article

Ribeiro et al Exp Neurol. 2022 Sep;355:114147. doi: 10

Insert

Ciliary neurotrophic factor (CNTF Human)

Use

AAV

Tags

HA-tag and NGF signal peptide

Expression

Mammalian

Promoter

UbC promoter with beta-globin intron

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

Plasmid

#113687

Purpose

SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 (NEWENTRY )

Use

AAV and CRISPR

Tags

Flag, HAx2, KASH, and NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

Plasmid

#113688

Purpose

SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 (NEWENTRY )

Use

AAV and CRISPR

Tags

Flag, HAx2, KASH, and NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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sgCRY1

Plasmid

#176511

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY1-2 (Cry1, Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Promoter

U6, hSyn

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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SIN-PGK-Cas9-V5-WPRE

Plasmid

#87876

Purpose

To produce lentiviral vector for Cas9 editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

spCas9

Use

Lentiviral

Tags

V5

Mutation

Human codon-optimized

Promoter

mouse PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP2

Plasmid

#178968

Purpose

Expresses miRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2

Use

AAV

Tags

no

Expression

Mammalian

Mutation

NO

Promoter

CAG

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS4-GCaMP6f

Plasmid

#178730

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE

Plasmid

#174134

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-dTom

Plasmid

#178717

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Tbg-CES2C-ΔC

Plasmid

#200559

Purpose

Secreted mouse CES2C driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)

Use

AAV

Tags

Flag

Promoter

TBG promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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scAAV-TTR-mFgf15

Plasmid

#190594

Purpose

AAV construct containing Fgf15 cds under control of TTR promoter

Depositor

Roel Nusse

Article

Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

Insert

Fgf15 (Fgf15 Mouse)

Use

AAV

Expression

Mammalian

Promoter

Ttr

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaCC-HA.SV40(polyA)

Plasmid

#155195

Purpose

Expresses murine Thrombospondin-1 (THBS1) protein with deletion of Coiled Coil domain and HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with deletion of amino acid residues 276-315

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-dTom

Plasmid

#178719

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS6-dTom

Plasmid

#178720

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of dTom in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS4-GFP

Plasmid

#178712

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Sox2 HMEJ donor

Plasmid

#97322

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Sox2. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Sox2 HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn iCyt_BrEt_SnFR_PM

Plasmid

#177757

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn iCyt_BrEt_SnFR_PM

Use

AAV

Promoter

hSyn

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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SIN-CMV-Cas9-V5-WPRE

Plasmid

#87904

Purpose

To produce lentiviral vector for gene editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

Cas9-V5

Use

Lentiviral

Tags

V5

Mutation

human codon-optimized

Promoter

CMV

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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