We narrowed to 63,680 results for: cloning
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Plasmid#90373PurposeHippo - TEAD1 gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterHippoAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_F753A_6xH
Plasmid#185710PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
Tags6xHis and MBPExpressionBacterialMutationChanged F753 to APromoterT7Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5-FRT/TO-3*Flag-APEX2 C-term NPAT
Plasmid#187585PurposeExpress FLAG epitope and APEX2-tagged NPAT fusion protein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNPAT (NPAT Human)
TagsAPEX2-FLAGExpressionMammalianPromoterCMVAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS
Plasmid#187588PurposeExpress FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS
TagsAPEX2-FLAGExpressionMammalianPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-CAAX
Plasmid#129285PurposeLentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequenceDepositorInsertFluorescent reporter mCherry fused to CAAX polybasic sequence
UseLentiviralTagsCAAXExpressionMammalianPromoterCMV promoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRE reporter backbone
Plasmid#158678PurposeThis plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberryDepositorTypeEmpty backboneUseLentiviralTagsmStrawberryExpressionMammalianAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOT_2 - lenti-EFS-tNGFR-2A-puro
Plasmid#181971PurposeExpresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.DepositorAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCEP4-puroeGFP
Plasmid#177922PurposeConstitutively expresses eGFP with puromycin selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
ExpressionMammalianPromoterCMVAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDY0973-RADARS iCaspase
Plasmid#182539PurposeExpress RADARS iCaspase that conditionally turn on iCaspase in the presence of target RNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiCaspase9 (CASP9 Human)
ExpressionMammalianPromoterEF1aAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShHELIX-KanR (CJT70)
Plasmid#181793PurposepDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nluc
Plasmid#180501PurposeMammalian expression of NlucDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNluc
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG654 pFAST
Plasmid#172861PurposeExpresses pFAST in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpFAST
TagsMyc tag (C terminal on insert)ExpressionMammalianPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9
Plasmid#175547PurposeCo-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-HaloTag7 and Tomm20-HaloTag9
ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE35
Plasmid#174409PurposeC. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
UseCRISPRExpressionBacterialAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPR-v2-HygR-EGFP
Plasmid#167188PurposeLentiviral expression of sgRNA with hygromycin resistance gene and EGFPDepositorInsertHygromycin B phosphotransferase and EGFP
UseCRISPR and LentiviralExpressionMammalianAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
7MEQ
Plasmid#176412PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertTMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
UseBaculovirus expresssionTags8 x His Tag and Honeybee melittin signalMutationetmprss2_S251D/R252D/Q253D/S254D/R255KPromoterPolyhedrinAvailable sinceOct. 21, 2021AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)
Plasmid#174515PurposeRecoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)DepositorInsertsMm PylS
MmtRNA-Pyl-opt(UGA)
ExpressionBacterialMutationASL from the serT gene (UGA anticodon) and recodedPromoterGlnS and lppAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDF0317 HvsCas7-11 mammalian expression
Plasmid#172511PurposeEncodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cellsDepositorInsertHvsCas7-11 mammalian expression
ExpressionMammalianAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v857.SGZ.codonopt
Plasmid#175182PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV hSyn FLEX iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianPromoterhuman SynapsinAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-miniAAVR-FLAG
Plasmid#166717PurposeVector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tagDepositorInsertminiAAVR-FLAG
UseLentiviralTagsFLAGExpressionMammalianMutationOnly expressing PKD1-3 of ectodomainPromoterCMVAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only