We narrowed to 59,448 results for: cloning

1. GGE253

   Plasmid

   #120790

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_GSY-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO HIS SSA1

   Plasmid

   #19543

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   SSA1 (SSA1 Budding Yeast)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSaI14

   Plasmid

   #8614

   Depositor

   Robert Hartley

   Article

   Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

   Insert

   Ribonuclease inhibitor SaI14
   

   Expression

   Bacterial

   Mutation

   N terminal threonine changed to alanine

   Available Since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pICSL80016

   Plasmid

   #50332

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS no stop codon, beta-glucuronidase (E. coli) with two introns
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICH72400

   Plasmid

   #50338

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   3'UTR, polyadenylation signal/terminator, Atug7 (A. tumefaciens)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3xFLAG-zDrosha

   Plasmid

   #171540

   Purpose

   Encodes Zebrafish Drosha protein

   Depositor

   Yan Zeng

   Article

   Li et al Biochem Biophys Res Commun. 2019 Jun 18;

   Insert

   drosha (rnasen Zebrafish)
   

   Expression

   Mammalian

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGT707

   Plasmid

   #51835

   Purpose

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   Tomato Mosaic virus 5'UTR+ CCAT
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH199_pE12_hyCal2-mCherry

   Plasmid

   #160412

   Purpose

   Gateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn system fused to mCherry

   Depositor

   Daniel Colón-Ramos

   Article

   Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

   Insert

   pDONR221 HyCal2::mCherry
   

   Use

   Other

   Tags

   mCherry

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHWBF01

   Plasmid

   #131022

   Purpose

   Synthetic source clone for maize Histon H2B.5 with C-terminal mCherry fusion

   Depositor

   Hank Bass

   Article

   Howe et al DNA Cell Biol. 2012 Jun;31(6):925-38. do

   Insert

   Histone H2B.5
   

   Use

   Cloning vector

   Tags

   mCherry

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-8xHis

    Plasmid

    #101699

    Purpose

    8xHis in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    AATG-[8xHis]-AGCC
    

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFOSGE_COF4D09

    Plasmid

    #70941

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    MED25 (MED25 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pGP8GF-ccdB

    Plasmid

    #188445

    Purpose

    Cloning Vector and pDEST

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    ccdB
    

    Expression

    Yeast

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFH85

    Plasmid

    #128198

    Purpose

    classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEnt R3L2-MCS

    Plasmid

    #26799

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    multiple cloning sequence
    

    Use

    Gateway entry vector

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGJJ055

    Plasmid

    #183622

    Purpose

    ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. GGE209

    Plasmid

    #120793

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp-Mfe
    

    Use

    Cloning/assembly plasmid

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBALU1

    Plasmid

    #69316

    Purpose

    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfp

    Depositor

    Oliver Hobert

    Article

    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

    Insert

    GFPint-FgF
    

    Expression

    Bacterial

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. GGE077

    Plasmid

    #120779

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Coding sequence of fluorescent protein in position 1 (G1_RedStarII)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    G1_RedStarII
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p667-UBC-TAOK1-V5-miniTurbo_IDG-K

    Plasmid

    #189887

    Purpose

    Gateway destination clone of TAOK1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TAOK1 (TAOK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. p667-UBC-AAK1-V5-TurboID_IDG-K

    Plasmid

    #135256

    Purpose

    Gateway destination clone of AAK1 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    AAK1-V5-TurboID (AAK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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