Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 11,800 results for: emb

Clear keywords
Showing: 5461 - 5480 of 11800 results

  5. PF3D7_0526900-COMP-blac-flag-his

    Plasmid
    #110985
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    transmembrane emp24 domain-containing protein, putative (PF3D7_0526900 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110985-plasmid-map-sequence-id-224476

    Enlarge

  6. PF3D7_0422100-COMP-blac-flag-his

    Plasmid
    #110984
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    transmembrane emp24 domain-containing protein, putative (PF3D7_0422100 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    110984-plasmid-map-sequence-id-224371

    Enlarge

  7. pANAP-P2A-eRF(E55D)

    Plasmid
    #241290
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses tRNACUA-Leu, AnapRS, and dominant negative ribosomal release factor
    Depositor
    Justin Taraska
    Publication
    Obashi et al Structure. Volume 34, Issue 1, 8 January
    Insert
    the amber suppressor tRNACUA-Leu
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV
    Available since
    Dec. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241290-plasmid-map-sequence-id-481082

    Enlarge

  9. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

    Plasmid
    #217767
    Purpose
    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Publication
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400
    Promoter
    T7 promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217767-plasmid-map-sequence-id-435788

    Enlarge

  10. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid
    #190598
    Purpose
    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter
    Depositor
    Claudia Rueckert
    Publication
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    mCherry - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190598-plasmid-map-sequence-id-379953

    Enlarge

  13. pelB-MBP-DipA-ss

    Plasmid
    #137068
    Purpose
    E. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature DipA: lacks the DipA signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137068-plasmid-map-sequence-id-291958

    Enlarge

  14. pelB-MBP-BB0323-ss

    Plasmid
    #137067
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0323: lacks the BB0323 signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137067-plasmid-map-sequence-id-291957

    Enlarge

  15. pKIF1C-BioID2-HA

    Plasmid
    #130980
    Purpose
    Expression of HA-tagged KIF1C-BioID2
    Depositor
    Anne Straube
    Publication
    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF1C-BioID2-HA (KIF1C Human)
    Tags
    BioID2-HA
    Expression
    Mammalian
    Mutation
    5 silent mutations that make this construct RNAi …
    Promoter
    CMV
    Available since
    Oct. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    130980-plasmid-map-sequence-id-259358

    Enlarge

  16. pVENUS-N1-FNTA

    Plasmid
    #86859
    Purpose
    To express FNTA with a Venus tag at C terminus.
    Depositor
    Yihong Ye
    Publication
    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FNTA (FNTA Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    The sequence of the insert is isoform 2, which di…
    Promoter
    CMV
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    86859-plasmid-map-sequence-id-175223

    Enlarge

  19. pSP64-hERG1a S624A

    Plasmid
    #53056
    Purpose
    Expresses alpha subunit of S624A hERG1a K channel in Xenopus oocytes
    Depositor
    Michael Sanguinetti
    Publication
    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    Use
    Xenopus oocyte expression
    Mutation
    changed Serine 624 to Alanine
    Promoter
    SP6
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53056-plasmid-map-sequence-id-82522

    Enlarge

Showing: 5461 - 5480 of 11800 results