We narrowed to 5,913 results for: pcDNA 3.1

1. NSP4-Δ220-234-Flag

   Plasmid

   #210341

   Purpose

   Expresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Δ220-234

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. NSP4-H120N/F121L-Flag

   Plasmid

   #210340

   Purpose

   Expresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H120N/F121L

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NSP4-FR-2Strep

   Plasmid

   #210316

   Purpose

   Expresses SARS-CoV-2 NSP4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CCM2_I432D_eGFP

   Plasmid

   #208879

   Purpose

   Express eGFP-CCM2 with the I432D mutation in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mutation I432D

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CCM2_W421AD422A_eGFP

   Plasmid

   #208880

   Purpose

   Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mutations W421A and D422A

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CCM2_I428S_eGFP

   Plasmid

   #208878

   Purpose

   Express eGFP-CCM2 with the I428S mutation in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mutation I428S

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. STIM1-2Strep

   Plasmid

   #210361

   Purpose

   Expresses STIM1 fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FKBP10-2Strep

   Plasmid

   #210357

   Purpose

   Expresses FKBP10 fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   FKBP10 (FKBP10 Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. DNAJC11-2Strep

   Plasmid

   #210354

   Purpose

   Expresses DNAJC11 fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   DNAJC11 (DNAJC11 Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dr-FGFR1

    Plasmid

    #184005

    Purpose

    Dr-FGFR expression in mammalian cells

    Depositor

    Vladislav Verkhusha

    Article

    Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

    Insert

    Dr-FGFR1 (FGFR1 Human)
    

    Use

    Tags

    Deinococcus radiodurans bacterial phytochrome

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS2308-Tier1-pGL3-PPGK-NLuc_TB

    Plasmid

    #169551

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRD400

    Plasmid

    #163107

    Purpose

    Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_eYFP_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRD188

    Plasmid

    #163099

    Purpose

    Expression of mEos3.2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mEos3.2_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. S113

    Plasmid

    #174551

    Purpose

    Constitutive PhCMV-driven mammalian expression vector of CDH-3-TF cassette (PhCMV-Gal4-mdm2-P2A-LD6-Rel65-pA)

    Depositor

    Bruno Correia

    Article

    Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

    Insert

    mdm2 and designed protein LD6 (MDM2 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGAL4-DBD-OLIG2-Adel-IDR

    Plasmid

    #145249

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and OLIG2-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    OLIG2-Adel-IDR (OLIG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 1010-AIDx-CP

    Plasmid

    #135340

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1010 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1010 nSpyCas9 -hAIDx
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 1058-AIDx-CP

    Plasmid

    #135342

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1058 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1058 nSpyCas9-hAIDx
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AIDx-ID-208

    Plasmid

    #135345

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 208 of nSpyCas9

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-208
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AIDx-ID-468

    Plasmid

    #135346

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 468 of nSpyCas9

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-468
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGAL4-DBD-HOXD11wt-IDR

    Plasmid

    #145242

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXD11wt-IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD11wt-IDR (HOXD11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge