We narrowed to 17,637 results for: She

1. pCAGEN-DV-IRES-myr-BFP (JDW 491)

   Plasmid

   #229834

   Purpose

   A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-phiC31o (JDW 453)

   Plasmid

   #229842

   Purpose

   A CMV-driven Phi31 recombinase.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   phiC31 codon optimized, PhiC31o
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-StayGoldc4-Giantin (JDW 1355)

   Plasmid

   #229844

   Purpose

   A gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent protein

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   n2StayGoldc4
   

   Use

   Gateway entry clone

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRII-dgat2 in situ probe

   Plasmid

   #232134

   Purpose

   zebrafish dgat2 anti-sense in situ probe, linearize with BamHI, T7 polymerase

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat2 (dgat2 Zebrafish)
   

   Use

   In vitro transcription

   Promoter

   SP6/T7

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ HA-BEX4

   Plasmid

   #231005

   Purpose

   Transient overexpression of human BEX4 in mammalian cells

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

   Insert

   BEX4 (BEX4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. CamKII-mtPyronicSF

   Plasmid

   #228906

   Purpose

   Expression of mtPyronicSF in neurons targeted to the mitochondria serving as an optical sensor for mitochondrial pyruvate uptake. Backbone from Addgene plasmid 100834

   Depositor

   Ghazaleh Ashrafi

   Article

   Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

   Insert

   pyronic SF
   

   Use

   AAV

   Tags

   Cox8 targetting

   Expression

   Mammalian

   Promoter

   CamKII

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

   Plasmid

   #223721

   Purpose

   Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   DGKD (DGKD Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted sterile alpha motif domain (deleted amino…

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3E-EF1a-pA (JDW 1222)

   Plasmid

   #224526

   Purpose

   A Gateway compatible 3' entry clone containing rat EF-1a polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EF-1a polyA
   

   Use

   Gateway cloning

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p40651-EFSNS-FMR1_e1-24xms2

   Plasmid

   #222968

   Purpose

   MS2 mRNA reporter with intact FMR1 5' UTR and exon 1 sequence

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   FMR1-31CGG 5' UTR and exon1
   

   Expression

   Mammalian

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E_hs_Glast (JDW 1182)

    Plasmid

    #224483

    Purpose

    Gateway compatible 5' entry clone containing the Human GLAST promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    GLAST promoter
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5E-hs_Cldn5 (JDW 1120)

    Plasmid

    #224481

    Purpose

    Gateway compatible 5' entry clone containing the Human Claudin 5 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Claudin-5 promoter
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME AmCyan (JDW 1151)

    Plasmid

    #224485

    Purpose

    Gateway compatible middle entry clone containing AmCyan cytosolic blue flourescent reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    AmCyan
    

    Use

    Gateway cloning

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME V5 mScarlet-I (JDW 1323)

    Plasmid

    #224487

    Purpose

    Gateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mScarlet-I
    

    Use

    Gateway cloning

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-LexA-mODC (no LexOp) (JDW 937)

    Plasmid

    #224504

    Purpose

    Gateway compatible middle entry clone containing a destabilized LexA transactivator

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-mODC
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-myc-BirA (JDW 1210)

    Plasmid

    #224509

    Purpose

    Gateway compatible middle entry clone containing a Myc tagged BirA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Myc-BirA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E-V5-mClover3-pA (JDW 1318)

    Plasmid

    #224530

    Purpose

    A Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mClover3-pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-V5-mTagBFP2 (JDW 871)

    Plasmid

    #224531

    Purpose

    A Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mTagBFP2
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)

    Plasmid

    #224537

    Purpose

    A Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    IRES-(n2)StayGold(c4)-pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-28b C7H1G6_6H N-terminal

    Plasmid

    #223434

    Purpose

    C7H1G6 GNAT expression vector with N-terminal His6 tag

    Depositor

    Emily Balskus

    Article

    Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

    Insert

    C7H1G6 GNAT
    

    Tags

    His6

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-28b R6CZ24_6H C-terminal

    Plasmid

    #223435

    Purpose

    R6CZ24 Thiolase expression vector with C-terminal His6 tag

    Depositor

    Emily Balskus

    Article

    Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

    Insert

    R6CZ24 Thiolase
    

    Tags

    His6

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald

    Plasmid

    #214468

    Purpose

    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S14D S25D S32D T261D

    Available Since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pILGFP4QE2Crimson

    Plasmid

    #218280

    Purpose

    Introduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    pURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
    

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. Human USP46

    Plasmid

    #217615

    Purpose

    Mammalian expression of USP46

    Depositor

    Ethan Lee

    Article

    Ng et al Nat Commun. 2023 Oct 5;14(1):6173. doi:

    Insert

    USP46 (USP46 Human)
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

    Plasmid

    #214471

    Purpose

    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S14D S25D S32D T261D

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR-Creb5(HA)

    Plasmid

    #195022

    Purpose

    Gateway vector containing HA-tagged Creb5

    Depositor

    Cheng-Hai Zhang, Andrew Lassar

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)
    

    Use

    Gateway cloning

    Tags

    3xHA

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GL4.10 E2E1- Prg4 promoter-luciferase

    Plasmid

    #195035

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Cheng-Hai Zhang, Andrew Lassar

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)
    

    Use

    Luciferase

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL4.10 E3E2E1-Prg4 promoter-luciferase

    Plasmid

    #195036

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Cheng-Hai Zhang, Andrew Lassar

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Prg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)
    

    Use

    Luciferase

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

    Plasmid

    #195037

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Cheng-Hai Zhang, Andrew Lassar

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)
    

    Use

    Luciferase

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. hIgG1-Ty1-hFc

    Plasmid

    #194593

    Purpose

    Mammalian expression of Ty1 nanobody upstream of human IgG1

    Depositor

    Gerald McInerney

    Article

    Hanke et al Nat Commun. 2020 Sep 4;11(1):4420. doi:

    Insert

    Ty1-hFc (S )
    

    Expression

    Mammalian

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Venus-Bik-del72-136-pEGFP-C3

    Plasmid

    #191411

    Purpose

    Transfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).

    Depositor

    David Andrews

    Insert

    Bcl-2-interacting killer (BIK Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    with deletion of amino acids 72-136 (del72-136) i…

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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31. pShuttle.Cre

    Plasmid

    #192930

    Purpose

    Gateway compatible vector with BsaI cloning site and Cre expression

    Depositor

    Thorsten Stiewe

    Article

    Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

    Type

    Empty backbone

    Use

    Gateway-compatible cloning vector

    Promoter

    CMV

    Available Since

    Dec. 9, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pv6_CBX7_2xChromo-W35A

    Plasmid

    #179397

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo(W35A)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_2xChromo(W35A)
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Chromodomain W35A

    Promoter

    CAGGS

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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33. pv6_CBX1_2xChromo-W42A

    Plasmid

    #179394

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo(W42A)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1_2xChromo(W42A)
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Chromodomain W42A

    Promoter

    CAGGS

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pv7_MBD1_MBD_noEGFP

    Plasmid

    #179406

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1_MBD
    

    Tags

    AviTag

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pv7_CBX1_2xChromo_noEGFP

    Plasmid

    #179395

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFP

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1_2xChromo_noEGFP
    

    Tags

    AviTag

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pv6_MBD1_MBD-R22A

    Plasmid

    #179405

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD(R22A)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1_MBD(R22A)
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    MBD R22A

    Promoter

    CAGGS

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEPQDKN0760

    Plasmid

    #185629

    Purpose

    Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T (BsaI-domesticated) which targets phytoene desaturase (PDS) of Nicotiana benthamiana

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEPQD0KN0751

    Plasmid

    #185628

    Purpose

    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    lacZ
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEPQD1CB0001

    Plasmid

    #185625

    Purpose

    MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. puc19_N_tag

    Plasmid

    #186650

    Purpose

    Empty donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    N-terminal 3xFlag-3xHA Tag
    

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge