We narrowed to 59,448 results for: cloning

1. pICH87611

   Plasmid

   #50284

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   Promoter and 5'UTR Ls (S.tuberosum)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH50581

   Plasmid

   #50256

   Depositor

   Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter, act2 (AT3G18780, Arabidopsis thaliana)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICH41402

   Plasmid

   #50285

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   5'UTR, omega (Tobacco Mosaic Virus)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR mCherry L1-R5

   Plasmid

   #43963

   Depositor

   Peter Hohenstein

   Article

   Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

   Insert

   mCherry
   

   Use

   Multisite gateway entr clone

   Available Since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFOSGE_COF3F03

   Plasmid

   #70962

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Utx (Utx Fly)
   

   Use

   Gateway entry vector

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pFH86

   Plasmid

   #128199

   Purpose

   improved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-Gal80

   Plasmid

   #51620

   Purpose

   vertebrate expression of full-length Gal80

   Depositor

   Joshua Bonkowsky

   Article

   Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

   Insert

   Gal80 (GAL80 Budding Yeast)
   

   Use

   Vertebrate expression

   Available Since

   March 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICSL30006

   Plasmid

   #50303

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS no stop codon, GFP (A. victoria)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-CMV-Flag-BRSK2_IDG-K

   Plasmid

   #135279

   Purpose

   Gateway destination clone of BRSK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

   Depositor

   Ben Major

   Insert

   Flag-BRSK2 (BRSK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBALU6

    Plasmid

    #69321

    Purpose

    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: Venus in frame w/ FRT-galK-FRT; no introns; N-terminal fusion

    Depositor

    Oliver Hobert

    Article

    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

    Insert

    Venus-FgF
    

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

11. SNAP-Paxillin

    Plasmid

    #186143

    Purpose

    For expresseion of recombinant paxillin in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PXN (PXN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Paxillin C1997A (silent mutation for cloning)

    Promoter

    Lac

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR eGFPCRE L1-R5

    Plasmid

    #43960

    Depositor

    Peter Hohenstein

    Article

    Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

    Insert

    eGFPCre
    

    Use

    Multisite gateway entr clone

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICH44199

    Plasmid

    #50286

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    5'UTR, omega (Potato Virus X)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pICH45125

    Plasmid

    #50261

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter, LHB1B1 (AT2g34430, A. thaliana)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKOdeltagalK (Apr)

    Plasmid

    #110091

    Purpose

    Analogous to pKO but without the negative selection gene galK.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKOdeltasacB (Kan)

    Plasmid

    #110092

    Purpose

    Analogous to pKO but without the negative selection gene sacB.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMEclecl14aP1gfp

    Plasmid

    #90123

    Purpose

    Gateway Mid-entry clone containing promoter sequence specific for clec14a driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    clec14aP1Egfp
    

    Tags

    EGFP

    Expression

    Bacterial

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p5Eclec14aE1

    Plasmid

    #90117

    Purpose

    5' Gateway entry clone containing endothelial specific enhancer sequence of clec14a

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    clec14aE1
    

    Expression

    Bacterial

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFH88

    Plasmid

    #128201

    Purpose

    sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GGE317

    Plasmid

    #120761

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 3 (P3_8UASxpr-TEF)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P3_8UASxpr-TEF
    

    Use

    Cloning/assembly plasmid

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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