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pFTK052

Plasmid

#171324

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Firefly luciferase reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK049

Plasmid

#171321

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

mCherry fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 22, 2021

Availability

Academic Institutions and Nonprofits only

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258/-2.5lacZ

Plasmid

#25429

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Development. 1995 Mar . 121(3):637-49.

Insert

Myo D Promoter (MYOD1 Human)

Use

Reporter construct

Tags

lacZ

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pHelper_O

Plasmid

#173783

Purpose

The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

186 Integrase

Use

Synthetic Biology

Expression

Bacterial

Mutation

E59G - please see depositor comments

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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TNXXL-CTS68

Plasmid

#48343

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

CTS68

Tags

TN-XXL

Expression

Mammalian

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pFTK051

Plasmid

#171323

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

eBFP fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGC630

Plasmid

#107013

Purpose

GFP reporter of lag-2 driven by 2 kb promoter

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Expression

Worm

Promoter

lag-2

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMN-326

Plasmid

#195451

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF3 BS - minTK - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_174

Plasmid

#199106

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pNG2 ORI plus NGS barcode 22, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_177

Plasmid

#199109

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBC1 ORI plus NGS barcode 25, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19/mlcR25-mEGFP

Plasmid

#154295

Purpose

Fluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP

Expression

Bacterial

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pRegev_ExonSkip_LandingPad

Plasmid

#204186

Purpose

Backbone for cloning individual exon sequences into beta globin minigene splicing reporter.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMB-PERr

Plasmid

#61203

Purpose

Fluorescent reporter for peroxisomes mCherry-SKL expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

mCherry-SKL

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAWH-Rluc-let-7-A114-N40-HhR

Plasmid

#50555

Purpose

Expression of luciferase reporter mRNA in S2 cell

Depositor

Yukihide Tomari

Article

Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

Insert

Luciferase-let7-A114-N40-HhR

Use

Luciferase

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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IRE-miR21-SunTag-TREAT

Plasmid

#196932

Purpose

SunTag cassette was added in the coding region in the miR21-IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pI12

Plasmid

#24273

Depositor

Mariano Garcia-Blanco

Article

Baraniak et al Mol Cell Biol. 2003 Dec . 23(24):9327-37

Insert

modified adenovirus exons and introns

Use

T7 for in vitro transcription

Expression

Mammalian

Available Since

March 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCMP-APr

Plasmid

#61184

Purpose

Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtPT4 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry

Tags

mCherry

Expression

Plant

Promoter

MtPT4

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-puro-BGHpA-TRE-pEF-tagRFP-T-NLS

Plasmid

#163083

Purpose

Donor vector for integration of TagRFP-T reporter at AAVS1 safe harbor site via TALENs

Depositor

Lacra Bintu

Article

Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

Insert

TagRFP-T

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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PacrAB-rfp + PmutS-yfp

Plasmid

#121442

Purpose

plasmid reporting the expression from the acrAB and mutS promoters

Depositor

Mary Dunlop

Article

El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

Inserts

PmutS-yfp
PacrAB-rfp

Use

Synthetic Biology; Pbb vector

Expression

Bacterial

Promoter

PacrAB and PmutS

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mScarlet

Plasmid

#191745

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mScarlet

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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