164,722 results

1. pelB-MBP-HtrA-ss

   Plasmid

   #137066

   Purpose

   E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
   

   Use

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature HtrA: lacks the HtrA signal peptide

   Promoter

   T7lac

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACEBac1-GCP2

   Plasmid

   #178068

   Purpose

   Insect cell expression of GCP2

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

   Insert

   GCP2 (TUBGCP2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRE-Tn5-163

   Plasmid

   #118542

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sYFP2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE/VEGF/IRES/EGFP

   Plasmid

   #206211

   Purpose

   An expression vector of VEGF and EGFP under control of TRE promoter

   Depositor

   Masamichi Kamihira

   Article

   Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

   Insert

   vascular endothelial growth factor (Vegfa Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

   Plasmid

   #170196

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

   Promoter

   CMV51

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CDC40

   Plasmid

   #155444

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   CDC40 (CDC40 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LCV2 V-1

   Plasmid

   #84804

   Purpose

   Cas9 with sgRNA targeting Exon 1 of VEGF-A

   Depositor

   Glenn Yiu

   Article

   Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

   Insert

   gRNA targeting VEGF-A (VEGFA Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcGFP-N1_CBP

   Plasmid

   #221903

   Purpose

   Transient expression of GFP-tagged CBP in mammalian cells

   Depositor

   Daniel Bose

   Insert

   CBP (CREBBP Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. XX01CAPN2A-c001

   Plasmid

   #175492

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   CAPN2 / CAPNS1 (CAPN2 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRE-Tn5-164

    Plasmid

    #118544

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mOrange2
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRE-Tn5-166

    Plasmid

    #118546

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC0042-PguCas13b crRNA backbone

    Plasmid

    #103853

    Purpose

    For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-PguCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSOX11.1.0-gDNA

    Plasmid

    #176378

    Purpose

    Expresses spCas9 and gRNA sequence targeting SOX11

    Depositor

    Barbara Stranger, Kevin White

    Insert

    SOX11 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBI-CMV1-mir451-CMV2-mir1

    Plasmid

    #164635

    Purpose

    Express miR-451 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR451a (MIR451A Human)
    miR-1-1 (MIR1-1 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPICZ-pre-Ost1-alphaf(I)-Btl2

    Plasmid

    #117667

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-alphaf(I)-Btl2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-Puro-TDP-43-WT

    Plasmid

    #133753

    Purpose

    Wild type human TDP-43 in pLVX-Puro lentiviral vector

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIBN-EYFP-TetR

    Plasmid

    #103798

    Purpose

    BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-TetR (CIB1, tetR Mustard Weed, E. coli)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-Cas9-NLS-6xHis

    Plasmid

    #62933

    Purpose

    Expression of Cas9-NLS-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9-NLS-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDM358_DPAKa(GBD)-YFP

    Plasmid

    #128700

    Purpose

    expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

    Depositor

    Igor Weber

    Article

    Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

    Insert

    GBD domain (aa 731-890) of DPAKa
    

    Use

    Dictyostelium discoideum expression vector

    Tags

    YFP

    Expression

    Mutation

    none

    Promoter

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. LCV2 V-3

    Plasmid

    #84806

    Purpose

    Cas9 with sgRNA targeting Exon 1 of VEGF-A

    Depositor

    Glenn Yiu

    Article

    Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

    Insert

    gRNA targeting VEGF-A (VEGFA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge