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pAT28-proD/SaEGFP

Plasmid

#120321

Purpose

Bacterial eGFP labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

eGFP with Staphylococcus aureus codon optimization

Expression

Bacterial

Promoter

proD

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57-GFP-2A-MCS

Plasmid

#71783

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSICO-CPSF6-eGFP

Plasmid

#167585

Purpose

Expresses human CPSF6 fused to eGFP

Depositor

Zandrea Ambrose

Article

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

CPSF6-eGFP (CPSF6 Human)

Use

Lentiviral

Tags

eGFP

Mutation

WT

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_550

Plasmid

#203398

Purpose

All-in-one lentiviral expression of gRNA and EnAsCas12a

Depositor

John Doench

Article

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Insert

Enhanced Acidaminococcus Cas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAdx-CMV-copGFP

Plasmid

#73346

Purpose

Express copGFP under CMV promoter

Depositor

Kazuhiro Oka

Insert

CMV-copGFP

Use

Adenoviral

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pp-mCAT1

Plasmid

#83948

Purpose

This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

Depositor

Vincent Lotteau, Philippe Mangeot

Article

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

mCAT1 (Slc7a1 )

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5_FRT_mCH

Plasmid

#127109

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

mCherry

Expression

Mammalian

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-NSP4SA11

Plasmid

#89171

Purpose

Express Rotavirus SA11 NSP4 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP4

Expression

Mammalian

Promoter

T7 promotor

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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ptf-Galectin3

Plasmid

#64149

Purpose

Expression in mammalian cells

Depositor

Tamotsu Yoshimori

Article

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin3 (LGALS3 Human)

Tags

EGFP and mRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8s-WPRE

Plasmid

#162374

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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LgBiT-KRAS (WT)_SmBiT-CRAF-RBD Bidirectional Vector

Plasmid

#237066

Purpose

Express LgBiT-KRAS (WT)_SmBiT-CRAF-RBD Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
cRAF(RBD) (RAF1 Human)

Tags

LgBiT and SmBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHIVT9-NAT-3xFlag-SMARCAL1-ΔN

Plasmid

#246936

Purpose

Lentiviral vector that expresses Flag-tagged SMARCAL1 with a deletion of the RPA-binding domain located in the N-terminal region (ΔN)

Depositor

Daniel Durocher

Article

Feng et al Mol Cell. 2024 Dec 5;84(23):4522-4537.e7

Insert

SMARCAL1 (SMARCAL1 Human)

Use

Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHIVT9-NAT-3xFlag-SMARCAL1-R764Q

Plasmid

#246937

Purpose

Lentiviral vector that expresses Flag-tagged SMARCAL1 with a mutation in the ATP translocase domain (R764Q)

Depositor

Daniel Durocher

Article

Feng et al Mol Cell. 2024 Dec 5;84(23):4522-4537.e7

Insert

SMARCAL1 (SMARCAL1 Human)

Use

Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTriEx-mVenus-Zdk1

Plasmid

#81047

Purpose

mammalian expression of Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-FOXK2

Plasmid

#153133

Purpose

Expression of Flag tagged human FOXK2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXK2 (FOXK2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-eGFP-Puro

Plasmid

#79823

Purpose

Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

Plasmid

#164611

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Expression

Mammalian

Mutation

S383T

Promoter

CMV

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAX APP Swe/Ind

Plasmid

#30145

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

human APP 695 Swedish/Indiana mutation (APP Human)

Expression

Mammalian

Mutation

Swedish and Indiana; K595N, M596L and V642F

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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FFA2-Tango

Plasmid

#66281

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

FFA2 (FFAR2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-NSP3SA11

Plasmid

#89170

Purpose

Express Rotavirus SA11 NSP3 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP3

Expression

Mammalian

Promoter

T7 promotor

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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