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pBabe puro-eIF4E

Plasmid

#33252

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

eIF4E (EIF4E Human)

Use

Retroviral

Tags

untagged

Expression

Mammalian

Mutation

unmodified

Promoter

SV40

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA 3.3 HA-hTSPAN12

Plasmid

#115784

Purpose

Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

TSPAN12 (TSPAN12 Human)

Tags

HA

Expression

Mammalian

Mutation

multiple silent mutations introduce restriction s…

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-K263E

Plasmid

#133758

Purpose

Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed lysine 263 to glutamate.

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot8-D40AE42A_U

Plasmid

#147647

Purpose

Mammalian Expression of HsNot8-D40AE42A

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsNot8-D40AE42A (CNOT8 Human)

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL11056

Plasmid

#68258

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Beta1-GFP pFB-Neo

Plasmid

#69767

Purpose

Expresses Beta1-GFP integrin for retroviral transfection

Depositor

Martin Humphries

Article

Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

Insert

Beta1 integrin (ITGB1 Human)

Use

Retroviral

Tags

GFP

Promoter

IRES

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
pSAM2_mCherry_Baf60c

Plasmid

#72691

Purpose

Doxycyline regulated expression of Baf60c in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Brg 1 (SMARCD3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.26-Ccnd2PromoterRegion1(1.2kb)

Plasmid

#228058

Purpose

1.2 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Ccnd2 promoter (Ccnd2 Mouse)

Use

Luciferase

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-HA-Chk2-D311V

Plasmid

#41921

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

Chk2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D311V

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pSAM2_mCherry_Tbx20

Plasmid

#72697

Purpose

Doxycyline regulated expression of Tbx20 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Tbx20 (Tbx20 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Grb10-WT

Plasmid

#74501

Purpose

Overexpression of Grb10 in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Mol Oncol. 2013 Jun;7(3):402-18. doi: 10

Insert

Grb10 (GRB10 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L358S, V522A, and V524L

Promoter

CMV

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCAX-EGFP-FBP17

Plasmid

#178365

Purpose

Expresses FBP17 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Expression

Mammalian

Promoter

Chicken Beta Actin

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCW-HA-hRb-WT-T373A-S608A-S612A-puro

Plasmid

#212674

Purpose

Express tagged hRb mutant

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

Rb (RB1 Human)

Use

Lentiviral

Tags

HA

Mutation

T373A/S608A/S612A

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A382T

Plasmid

#133756

Purpose

Lentiviral expression of human TDP-43 A382T disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Mutation

Changed alanine 382 to threonine

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Hchk2 (kin-)-PUNI10

Plasmid

#41908

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Expression

Bacterial

Mutation

kinase dead (D347A)

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x

Plasmid

#182256

Purpose

Human PDAP1 3` UTR region mutated in 4x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene mutated in 4 binding sites for mir-150

Use

Luciferase

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL-V5

Plasmid

#204531

Purpose

Mammalian expression of human MPL-V5

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-V5 (MPL Human)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-ICP0-FxE

Plasmid

#235539

Purpose

To express tagBFP-2A-ICP0-FXE (ICP0 ∆106-149)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0-FxE

Use

Lentiviral

Mutation

Deletion of the RING domain, ∆106-149.

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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ins:GCaMP6s; cryaa:RFP

Plasmid

#110285

Purpose

A zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Insert

GCaMP6s

Use

Zebrafish

Promoter

insulin

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-YY1

Plasmid

#154943

Purpose

Knocking down of human YY1 through shRNA and amiRNA targeting YY1

Depositor

Claes Wadelius

Article

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

shRNA and amiRNA against YY1 (YY1 Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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