We narrowed to 7,184 results for: RAP

1. pPB-EF1A-HALO-hLRRK2

   Plasmid

   #229738

   Purpose

   Expresses Halo tagged human LRRK2 in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MFE23-ZZ

   Plasmid

   #223553

   Purpose

   MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.

   Depositor

   Jean Gariepy

   Article

   Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

   Insert

   pET302-MFE23-ZZ
   

   Use

   Tags

   HA tag and HIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas3-APOBEC1

   Plasmid

   #229536

   Purpose

   pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   rAPOBEC1
   Cas3
   Uracil glycosylase inhibitor
   

   Use

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Promoter

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

   Plasmid

   #225228

   Purpose

   Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

   Depositor

   Urartu Seker

   Article

   Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

   Inserts

   Aga2
   Htt (25Q)
   

   Use

   Tags

   HA Tag and c-myc Tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

   Plasmid

   #225229

   Purpose

   Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

   Depositor

   Urartu Seker

   Article

   Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

   Inserts

   Aga2
   Htt (46Q)
   

   Use

   Tags

   HA Tag and c-myc Tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_FLAG_hSF3B1

   Plasmid

   #176590

   Purpose

   Adenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1

   Depositor

   Manoj Pillai

   Article

   Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

   Insert

   SF3B1 (SF3B1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Control-Control-LRG-GFP

   Plasmid

   #225873

   Purpose

   Two copies of guide RNA targeting a control sequence

   Depositor

   Janai Carr-Ascher

   Article

   Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

   Insert

   Control
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. RNF149 shRNA-TRE

   Plasmid

   #225337

   Purpose

   shRNAmir backbone under tet-operator for RNA interference, with YFP

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   RNF149 shRNA (Rnf149 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (tetracycline-responsive element)

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-MCHpr-GCaMP6f

   Plasmid

   #221039

   Purpose

   Expresses GCaMP6f under the rat pro-melanin-concentrating hormone promoter

   Depositor

   Yanhua Huang

   Article

   Guo et al Biol Psychiatry. 2022 Dec 1;92(11):880-8

   Insert

   GCaMP6f
   

   Use

   AAV; Rat targeting

   Tags

   Expression

   Mammalian

   Mutation

   A317E

   Promoter

   Rat MCH

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)

    Plasmid

    #206434

    Purpose

    Vasa 463-661 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Vasa 463-661 (vas Fly)
    

    Use

    Tags

    EGFP

    Expression

    Insect

    Mutation

    F504E

    Promoter

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1-PH-mCherry-Oskar 139-240 MUT (A162E/L228E) (HK202)

    Plasmid

    #206464

    Purpose

    Oskar 139-240 mut expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Oskar 139-240 mut (osk Fly)
    

    Use

    Tags

    PH mcherry

    Expression

    Insect

    Mutation

    A162E L228E)

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-cT3G-cERP2-SOX17

    Plasmid

    #222925

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of SOX17

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PiggyBac EF1a-eGFP-Puro U6 MMP2 g1

    Plasmid

    #170824

    Purpose

    PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d MMP2 gRNA1
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PiggyBac EF1a-eGFP-Puro U6 MMP2 g2

    Plasmid

    #170825

    Purpose

    PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d MMP2 gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PiggyBac EF1a-eGFP-Puro U6 ANXA2 g1

    Plasmid

    #170826

    Purpose

    PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d ANXA2 gRNA1
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PiggyBac EF1a-eGFP-Puro U6 ANXA2 g2

    Plasmid

    #170827

    Purpose

    PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d ANXA2 gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PiggyBac EF1a-eGFP-Puro U6 CLCN3 g1

    Plasmid

    #170828

    Purpose

    PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d CLCN3 gRNA1
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2

    Plasmid

    #170829

    Purpose

    PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d CLCN3 gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAVS1-Puro CAG eGFP NLS

    Plasmid

    #170830

    Purpose

    Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    eGFP
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PiggyBac EF1a-eGFP-Puro U6 PPP2CA g1

    Plasmid

    #170822

    Purpose

    PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d PPP2CA gRNA1
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge