We narrowed to 8,320 results for: Set

1. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131462

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   H3F3A K27M, Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131463

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   H3F3A G34R, Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-TAC-EB-C5

   Plasmid

   #80485

   Purpose

   piggyBac transposon for dox-inducible expression of the EB-C5 polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   lin-28 homolog A (C. elegans) (Pou5f1, Sox2, Klf4, Myc, Lin28a Mouse)
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

   Plasmid

   #129420

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   TagBFP2-V5 tag

   Expression

   Mutation

   G12V

   Promoter

   none (3x polyA to mitigate episomal expression)

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EC71174

   Plasmid

   #154067

   Purpose

   Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Inserts

   HS Cre recombinase
   Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
   HYGROMYCIN Resistance Cassette
   

   Use

   Cre/Lox and Synthetic Biology; Golden gate

   Tags

   Expression

   Bacterial and Plant

   Mutation

   The Arabidopsis thaliana U5 small nuclear ribonuc…

   Promoter

   35S promoter, Hordeum vulgare HSP 17 promoter use…

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUAP1

   Plasmid

   #63674

   Purpose

   Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.

   Depositor

   Nicola Patron

   Article

   Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np

   Insert

   RFP cloning selection cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

   Plasmid

   #131730

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   TagBFP2-V5 tag

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-EEA-5g-OSK2M2L1-PGK-Puro

   Plasmid

   #102909

   Purpose

   PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. GG-EBNA-OMKSL-PP

   Plasmid

   #102899

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OMKSL-gRNAS-PGK-puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AiP13640: pAAV-AiE0949h-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3640)

    Plasmid

    #230864

    Purpose

    AiE0949h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal neuron subset

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    SYFP2-P2A-3XFLAG-10aa-H2B
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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