We narrowed to 15,732 results for: LEA

1. pLPCX cyto Grx1-roGFP2

   Plasmid

   #64975

   Purpose

   Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-SpCas9-NG

   Plasmid

   #117919

   Purpose

   Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

   Depositor

   Osamu Nureki

   Article

   Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

   Insert

   SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
   

   Expression

   Mammalian

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPPED

   Plasmid

   #162468

   Purpose

   Destination Expression vector for dicot plant prime editing

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Inserts

   Cas9H840A
   attR gateway destination cassette
   

   Tags

   MLV reverse transcriptase

   Expression

   Plant

   Mutation

   Change histidine 840 to Alanine based sequence o…

   Promoter

   2x35S

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CT691

   Plasmid

   #233660

   Purpose

   BLADE (two-input, four-output decoder); Cre excises lox sites to express GFP, Flp excises FRT sites to express iRFP, and the presence of both recombinases is designed to express mRuby.

   Depositor

   Wilson Wong

   Article

   Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

   Insert

   BLADE
   

   Expression

   Mammalian

   Mutation

   None

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiGuide

   Plasmid

   #117986

   Purpose

   pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.

   Depositor

   Paul Khavari

   Article

   Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

   Insert

   sgRNA backbone (F+E)
   

   Use

   Lentiviral

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-NE2m-IRES-mCherry-CAAX

   Plasmid

   #208690

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. HBM-Luc

   Plasmid

   #35155

   Depositor

   Linda Penn

   Article

   Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

   Insert

   Myc promoter (MYC Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Contains 2.5 kb of the c-myc minimal promoter

   Promoter

   c-myc

   Available Since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEasiLV-MCS-coICP0

   Plasmid

   #235545

   Purpose

   To express ICP0 (doxycycline inducible)

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

   Insert

   ICP0
   

   Use

   Lentiviral

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. cycLuc_TEVS

   Plasmid

   #119207

   Purpose

   Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   cyclic luciferase with TEVs cleavage site
   

   Use

   Luciferase

   Tags

   6xHis and AU1 tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1 hPGK-PuroR-pA donor

    Plasmid

    #22072

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    PGK-Puro (PGK1 Human)
    

    Use

    Zfn donor

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-sL7-Cre-HA-WPRE

    Plasmid

    #204488

    Purpose

    For production of driver AAV virus for Purkinje cell-specific expression

    Depositor

    Samuel Wang

    Article

    Chen et al Nature. 2022 May;605(7911):722-727. doi:

    Insert

    Cre
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Promoter

    sL7

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. xCas9

    Plasmid

    #138565

    Purpose

    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    xCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDisplay-NE2h-IRES-mCherry-CAAX

    Plasmid

    #208691

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cells

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SuExp-hPLD4

    Plasmid

    #201244

    Purpose

    express full length human PLD4 protein

    Depositor

    David Nemazee

    Article

    Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

    Insert

    PLD4 (PLD4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGPTVII-SED1

    Plasmid

    #232615

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 Biosensor
    

    Expression

    Plant

    Promoter

    pUBQ10

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR_L1-YFP-Turbo-L2

    Plasmid

    #127350

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

    Plasmid

    #107407

    Purpose

    Lentiviral expression of gRNA resistant PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    sg resistant PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Mutation

    sgRNA resistant

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. shPBRM1#32-Tet-pLKO-puro

    Plasmid

    #107399

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. HMBP-3C-STAR.66-285

    Plasmid

    #100094

    Purpose

    expresses MBP-tagged STARD1 protein domain (residues 66-285)

    Depositor

    Nikolai Sluchanko

    Article

    Sluchanko et al Protein Expr Purif. 2016 Mar;119:27-35.

    Insert

    STAR protein (STAR Human)
    

    Tags

    His-tagged MBP

    Expression

    Bacterial

    Promoter

    Ptac

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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