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We narrowed to 13,953 results for: LEA

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Showing: 5541 - 5560 of 13953 results

  2. pBTK601

    Plasmid
    #110607
    Purpose
    BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)
    Depositor
    Jeffrey Barrick
    Publication
    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    Synthetic Biology
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    110607-plasmid-map-sequence-id-215544

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  4. pCAG-T3-eSpCAS9-NG-pA

    Plasmid
    #131466
    Purpose
    Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized eSpCas9-NG
    Use
    CRISPR
    Tags
    Flag, NLS, and polyA
    Expression
    Mammalian
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131466-plasmid-map-sequence-id-262445

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  5. pAAV-hSyn-ChRger1-TS-EYFP

    Plasmid
    #127243
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.
    Depositor
    Viviana Gradinaru
    Publication
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger1-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Promoter
    hSyn1
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127243-plasmid-map-sequence-id-247230

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  6. pAAV-CaMKIIa-ChRger3-TS-EYFP

    Plasmid
    #127241
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Publication
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger3-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Promoter
    CamKIIa
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127241-plasmid-map-sequence-id-247096

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  7. pCRIS

    Plasmid
    #120424
    Purpose
    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.
    Depositor
    Tamás Fehér
    Publication
    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:
    Insert
    CRISPR spacers targeting IS1, IS5, IS3, IS150
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    constitutive
    Available since
    Jan. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    120424-plasmid-map-sequence-id-233246

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  8. pOCC167

    Plasmid
    #118909
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118909-plasmid-map-sequence-id-232266

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  16. pLNT-SFFV-mRuby2

    Plasmid
    #87217
    Purpose
    lentiviral expression of mRuby2
    Depositor
    Violaine Sée
    Insert
    mRuby2
    Use
    Lentiviral
    Tags
    6xHis-T7
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    87217-plasmid-map-sequence-id-173864

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  18. GALTA-c001

    Plasmid
    #79645
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GALT (GALT Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79645-plasmid-map-sequence-id-150209

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  20. pFLIP38

    Plasmid
    #65616
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Publication
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Tags
    6His-edCit-attR1 and attR2-edCer-cMyc
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65616-plasmid-map-sequence-id-111417

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Showing: 5541 - 5560 of 13953 results