We narrowed to 16,387 results for: LEA
-
Plasmid#184981PurposeExpress -Eco1 FAA1 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
ExpressionYeastMutationFAA1 donor P233stopPromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.113
Plasmid#184982PurposeTest effect of extending a1/a2 on FAA1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
ExpressionYeastMutationFAA1 donor P233stop, a1/a2 length extended to 27 ā¦PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.002
Plasmid#184972PurposeExpress -Eco1 ADE2 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
ExpressionYeastMutationADE2 donor P272stopPromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.110
Plasmid#184979PurposeExpress -Eco1 TRP2 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
ExpressionYeastMutationTRP2 donor E64stopPromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.039
Plasmid#184973PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
ExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 ā¦PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
hsLEMD2-pX330
Plasmid#179511PurposeEncodes gRNA for human LEMD2DepositorInsertgRNA for LEMD2
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7
Plasmid#186753PurposeA basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.DepositorTypeEmpty backboneUseSynthetic Biology; Cloning backbonePromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-NLSlacZ-stop-L2
Plasmid#186358PurposeEntry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ Synthetic, E. coli)
UseExpression of nuclear lacz reporterTagsNuclear localization signalAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.407
Plasmid#187204PurposeExpresses retron Eco1 ncRNA v32, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v32
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.408
Plasmid#187205PurposeExpresses retron Eco1 ncRNA v35, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB556
Plasmid#185108Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160ADepositorInsertPOM152
TagsGFPExpressionYeastMutation1st 200 amino acids (N-term + transmembrane domaiā¦Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB438
Plasmid#185092PurposeGAL1::Rfa1[1-337, 344-621]-GFP with NLS removedDepositorInsertRFA1
ExpressionYeastMutationRfa1 with amino acids 338-343 deletedPromoterGAL1Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB372
Plasmid#185084PurposeGAL::Rfa1[330-621]-GFP under control of GAL1 promoterDepositorInsertRFA1
TagsGFPExpressionYeastMutationRfa1 amino acids 330-621PromoterGAL1Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)
Plasmid#158771PurposeExpression of synaptotagmin 1 variant with self-cleaving C2 domain.DepositorInsertSynaptotagmin 1 1-NS3/4a (Syt1 Mouse)
UseLentiviralMutationFlexible linkers, ADVVCC/QMSY cleavage siteAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
NCH2exp
Plasmid#131019PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH2
TagseGFP-FLAG-HAExpressionPlantPromoterP35SAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MNEAP1ec
Plasmid#131020PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMNEAP1
UseGateway CloningTagseGFP-FLAG-HAAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MNEAP1exp
Plasmid#131021PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMNEAP1
TagseGFP-FLAG-HAExpressionPlantPromoterP35SAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MKAKU41ec
Plasmid#131014PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMKAKU41
UseGateway CloningTagsmCherry-FLAG-HAAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
MKAKU41exp
Plasmid#131015PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMKAKU41
TagsmCherry-FLAG-HAExpressionPlantPromoterP35SAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
NCH1ec
Plasmid#131016PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH1
UseGateway CloningTagseGFP-FLAG-HAAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits