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pN1-TOMM20-Halo

Plasmid

#129348

Purpose

Halo Tag, Mitochondrial outer membrane localization

Depositor

Yasushi Okada

Article

Wang et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

Halo (Promega)

Expression

Mammalian

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0112

Plasmid

#177044

Purpose

MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol synthase (CrGES) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0818

Plasmid

#177020

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geranyl pyrophosphate synthase; geranyl diphosphate synthase

Use

Synthetic Biology

Mutation

transit peptide removed

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pTK-EdnrbE2-AVI-Tfap2B-tev-FLAG-2A-Citrine

Plasmid

#127776

Purpose

Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Tfap2B in frame with an Avi tag at the N terminus and FLAG-tag and Citrine at the C-terminus

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

tfap2b (TFAP2B Chicken)

Use

Unspecified

Tags

Avi

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF5

Plasmid

#117157

Purpose

Target site TATTTCCCTGTCTCCTTGGCC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF7

Plasmid

#117158

Purpose

Target site ATAAGGAAGCACTCGATGTCG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a T7pCONS TIR-2 sfGFP

Plasmid

#154464

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_PGK

Plasmid

#79120

Purpose

Lentiviral vector for constitutive expression of genes (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

Plasmid

#20945

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

hSyn

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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1098 pcDNA GFP FKHR

Plasmid

#9022

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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1136- Desmoplakin-GFP

Plasmid

#32227

Depositor

Kathleen Green

Article

Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

Insert

Desmoplakin (DSP Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pGL0_12 [pAMβ1 ORI]

Plasmid

#198926

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAMβ1 ORI

Use

Synthetic Biology

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_NUP98_HOXD13

Plasmid

#205871

Purpose

Express mEGFP-tagged fusion protein, NUP98_HOXD13 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NUP98_HOXD13 (NUP98, HOXD13 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-ARHGEF2

Plasmid

#207959

Purpose

Expression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry tag

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

ARHGEF2 (ARHGEF2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7 RNA POL

Plasmid

#138352

Purpose

Expressing T7 RNA Polymerase in mammalian cells

Depositor

Baisong Lu

Insert

T7 RNA POL

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-hGSDMD

Plasmid

#111559

Purpose

Express human GSDMD in E.coli

Depositor

Hongbo Luo

Article

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (GSDMD Human)

Tags

His, SUMO

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pFCIV-CEACAM6

Plasmid

#149303

Purpose

lentiviral packaging vector for CEACAM6 expression.

Depositor

James Fleckenstein

Article

Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

Insert

CEACAM6 (CEACAM6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

ubiquitin C

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ142

Plasmid

#69294

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_PsCatCh2.0-YFP

Plasmid

#186978

Purpose

Expresses PsCatCh2.0 channelrhodopsin in mammalian cells

Depositor

Adam Cohen

Article

Hayward et al eLife. 2023 Oct 6;12:RP86833. doi: 10.75

Insert

PsCatCh2.0-YFP

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pKDEL-mScarlet3-H (aka mYongHong)

Plasmid

#239411

Purpose

Expresses mScarlet3-H (aka mYongHong) localized to ER in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

mScarlet3-H-KDEL

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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