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pLenti PGK mKate2-OGT WT (Puro)

Plasmid

#154290

Purpose

Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x FLAG and mKate2

Expression

Mammalian

Mutation

WT

Promoter

PGK

Available Since

Dec. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pInducer20 ALPK1 Y254C

Plasmid

#231587

Purpose

ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

ALPK1 (ALPK1 Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

changed tyrosine 254 to cysteine

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-UBR5

Plasmid

#231238

Purpose

Flag-tagged wild-type UBR5 over-expression construct for transient expression in mammalian cells

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

UBR5 (UBR5 Human)

Expression

Mammalian

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-NGFR

Plasmid

#164520

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 ALPK1 V1092A

Plasmid

#231588

Purpose

ALPK1 gene mutated in position V1092A (mutation found in spiradenomacarcinoma (sweat gland cancer)) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

ALPK1 (ALPK1 Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

changed Valine1092 to Alanine

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 ALPK1 T237M

Plasmid

#231586

Purpose

ALPK1 gene mutated in position T237M (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

ALPK1 (ALPK1 Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

changed threonine 237 to Methionine

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SV40NLSf-mScarletP2A-hLuc

Plasmid

#218793

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

NLSf-mScarletP2A-hLuc

Use

AAV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgfiller-eCas9-T2A-BlastR

Plasmid

#172437

Purpose

Expresses eCas9, BlastR and sgRNA cloning site

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

eCas9
BlastR

Use

CRISPR, Lentiviral, and Mouse Targeting

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS2241

Plasmid

#111896

Purpose

ssAAV genome with Ple320 (POU4F1 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple320 (POU4F1 Human)

Use

AAV

Promoter

Ple320 (POU4F1 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-EZH2-PGK-Puro-IRES-GFP

Plasmid

#75125

Purpose

Retroviral expression plasmid encoding wild type human EZH2

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
CaMKII-somBiPOLES-mCerulean

Plasmid

#154948

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde, AAV5, and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

CaMKIIa(0.4)

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-REST_sh_seq1_WPRE

Plasmid

#127573

Purpose

shRNA to knock down RE1-silencing transcription factor (REST)

Depositor

Malin Parmar

Article

Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

Insert

shRNA for knock down of REST (REST Human)

Use

Lentiviral

Promoter

U6

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-human HoxB4-IRES-YFP

Plasmid

#91889

Purpose

Retroviral expression of human HoxB4

Depositor

Martine Roussel, Brian Sorrentino

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

HoxB4 (HOXB4 Human)

Use

Retroviral

Tags

IRES-EYFP

Expression

Mammalian

Promoter

MMLV gag

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCas-pML-1B_6xHis-Cas12m

Plasmid

#192282

Purpose

MmCas12m fused to His-tag for protein purification

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12m

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS2273

Plasmid

#111899

Purpose

ssAAV genome with Ple343 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple343 (TUBB3 Human)

Use

AAV

Promoter

Ple343 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS2280

Plasmid

#111901

Purpose

ssAAV genome with Ple345 (NEFL MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple345 (NEFL Human)

Use

AAV

Promoter

Ple345 (NEFL MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1REST_sh_seq2_WPRE

Plasmid

#127574

Purpose

shRNA to knock down RE1-silencing transcription factor (REST)

Depositor

Malin Parmar

Article

Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

Insert

shRNA for knock down of REST (REST Human)

Use

Lentiviral

Promoter

U6

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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hV1aR-GFP

Plasmid

#67846

Purpose

mammalian expression of human V1a receptor with GFP tag

Depositor

Christian Gruber

Article

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

V1a receptor (AVPR1A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
hV1b-GFP-1

Plasmid

#67847

Purpose

mammalian expression of human V1b receptor with GFP tag

Depositor

Christian Gruber

Article

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

V1b receptor (AVPR1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
hU6-pegRNA-EF-1α-Cre Template

Plasmid

#196036

Purpose

The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

Depositor

Tyler Jacks

Article

Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

Type

Empty backbone

Expression

Mammalian

Promoter

hU6 and EF-1-alpha

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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