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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Expression

Worm

Promoter

rab-3

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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UbG76V-eGFP-V5His_20aa

Plasmid

#23970

Depositor

Nico Dantuma

Article

Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

Insert

Ub-G76V-eGFP-V5HIS_20aa

Tags

eGFP

Expression

Mammalian

Mutation

G76V mutation in Ubiquitin. Ub fused to N-termin…

Available Since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

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AmCyan-P2A-mCherry

Plasmid

#45350

Depositor

Ilpo Huhtaniemi

Article

Potorac et al J Endocrinol. 2016 Sep 21. pii: JOE-16-0

Insert

AmCyan

Tags

mCherry

Expression

Mammalian

Mutation

SV40 NLS

Promoter

CMV

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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prhaBAD-anti-rabbit-Nb-Hia5

Plasmid

#239627

Purpose

Rhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferase

Depositor

Aaron Straight

Article

Sidhwani et al bioRxiv 2025.07.01.662447; doi: https://

Insert

Hia5

Tags

6HIS, MBP, TEV, and anti-Rabbit Nanobody

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

rhaBAD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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eSrtA(2A-9) pET29b

Plasmid

#75145

Purpose

reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

Depositor

David Liu

Article

Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

reprogrammed sortase A variant

Tags

6x His

Expression

Bacterial

Mutation

S102C A104H E105D K138P K152I N160K K162H T164N K…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only

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2xMyc-tdMCP-SNAP (tdMCP standard)

Plasmid

#226291

Purpose

Lentiviral plasmid expressing a tdMCP-protein standard. Used in LABEL-seq abundance assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

2xMyc-tdMCP-SNAPtag
EGFP-IRES-BlastR

Use

Lentiviral

Tags

2xMyc-tdMCP

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-TRBP

Plasmid

#50351

Purpose

bacterial expression of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

Insert

TRBP (TARBP2 Human)

Tags

His and Myc

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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mNeongreen-2xrGBD

Plasmid

#129624

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

mNeongreen-2xrGBD

Expression

Mammalian

Promoter

CMVdel

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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2xMyc-tdMCP-SNAP (tdMCP standard)

Plasmid

#226166

Purpose

For transient expression of the tdMCP-protein standard. Used in LABEL-seq abundance assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

2xMyc-tdMCP-SNAPtag

Use

Lentiviral

Tags

2xMyc-tdMCP and EGFP-IRES-BlastR

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp2

Plasmid

#163587

Purpose

Expresses BMP-2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp2 (Bmp2 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pIVT-dCas9-mCherry

Plasmid

#174443

Purpose

in vitro transcription template for dCas9-mCherry

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Cas9 Endonuclease Dead

Use

In vitro transcription template

Tags

mCherry

Promoter

T7 SP6

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mCardinal-N1

Plasmid

#54590

Purpose

Localization: N1 Cloning Vector, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

mCardinal

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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XE239 Fz1-ER

Plasmid

#16816

Depositor

Randall Moon

Article

Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

Insert

Fz1-ER (Fzd1 Rat)

Use

Xenopus expression

Available Since

May 19, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-PACT

Plasmid

#50352

Purpose

bacterial expression of PACT

Depositor

Kumiko Ui-Tei

Article

Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

Insert

PACT (PRKRA Human)

Tags

His and Myc

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNox4

Plasmid

#69353

Purpose

expresses mouse Nox4 in mammalian cells

Depositor

Karl-Heinz Krause

Article

Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

Insert

mNox4 (Nox4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
pNDL-60

Plasmid

#161720

Purpose

In vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA

Depositor

Adam Cohen

Article

McNamara et al bioRxiv [Preprint]. 2024 Apr 12:2024.04.

Insert

cytosolic acvr2b-CIB1N (acvr2b Zebrafish)

Use

In vitro transcription

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBT277

Plasmid

#122608

Purpose

express evoCDA1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoCDA1-BE4max

Expression

Mammalian

Mutation

F23S A123V I195F

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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LXRalpha_pcDNA3.1

Plasmid

#177621

Purpose

Full-length human LXR alpha cDNA sequence in pcDNA3.1+

Depositor

Thomas Burris

Article

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

LXR alpha (NR1H3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-UTY-F

Plasmid

#40617

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

UTY (UTY Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-TRAIL

Plasmid

#10953

Depositor

Bingliang Fang

Article

Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

Insert

Tumor necrosis factor alpha related apoptosis inducing ligand (TNFSF10 Human)

Tags

GFP

Expression

Mammalian

Mutation

NA

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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