We narrowed to 10,337 results for: plasmids 101

1. pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

   Plasmid

   #183069

   Purpose

   Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c_4
   

   Use

   CRISPR

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   Wild-type

   Promoter

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCX-Klf4

   Plasmid

   #66656

   Purpose

   For expression of mouse Klf4.

   Depositor

   Barak Cohen

   Article

   Fiore et al Genome Res. 2016 Jun;26(6):778-86. doi:

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

   Plasmid

   #124861

   Purpose

   Mutagenesis of Slc17a8

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a8 (Slc17a8 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNB23

   Plasmid

   #103058

   Purpose

   medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGp

   Depositor

   Dirk Bumann

   Article

   Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

   Insert

   GFP-OVA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hydrogen peroxide-inducible promoter katGp

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-LAP1_2-mCherry

   Plasmid

   #159526

   Purpose

   Expresses mCherry from LAP1_2 promoter

   Depositor

   Esteban Engel

   Article

   Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LAP1_2

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. DNA-CARtcr-IRESpuro

   Plasmid

   #89346

   Purpose

   2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette

   Depositor

   Ron Vale

   Article

   Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

   Insert

   TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
   

   Use

   Lentiviral

   Tags

   SNAPf tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pG23A

   Plasmid

   #102884

   Purpose

   Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
   

   Use

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Native promoter

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSR02

   Plasmid

   #69149

   Purpose

   Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Inserts

   mCherry
   eGFP
   

   Use

   Cre/Lox; Mossci

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0

   Promoter

   rps-27

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGCNK58

   Plasmid

   #202461

   Purpose

   Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells

   Depositor

   Stephen Harrison, Arthur Palmer

   Article

   Weiss et al Nature. 1990 Oct 11;347(6293):575-8. doi

   Insert

   Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA3_GAL4UAS-Luciferase reporter

    Plasmid

    #64159

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA3 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV Fos YFP MS2

    Plasmid

    #131775

    Purpose

    Fos upstream msfYFP 24xMS2 Fos downstream for CRISPR tagging

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    msfYFP - 24x MS2 loops
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-DIO {ChETA}on-WPRE

    Plasmid

    #111386

    Purpose

    AAV expression of ChETA (hChR2 with E123T/H134R mutations) fused to HA tag driven by hSynapsin promoter in Cre-ON manner for optogenetic activation

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Insert

    ChETA
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    humanized ChR2, E123T/H134R

    Promoter

    hSynapsin

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSR29

    Plasmid

    #69255

    Purpose

    pelt-2::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0, tbb-2 UTR is in wrong o…

    Promoter

    elt-2

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR_PGK_SNIPR_synRobo/Notch

    Plasmid

    #188341

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Robo1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_Robo1-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CoV-NSP3-c001

    Plasmid

    #173082

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E1000-K1173, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-U6-sgRNA-PGK-puromycin-AflII

    Plasmid

    #106404

    Purpose

    Empty guide RNA cloning vector with an AflII site added from Addgene 41824

    Depositor

    Jason Gertz

    Article

    Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLXIN-Ap1g1-FKBP

    Plasmid

    #46946

    Purpose

    Retroviral plasmid for co-expressing siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cells

    Depositor

    Margaret Robinson

    Article

    Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

    Insert

    Ap1g1-FKBP
    

    Use

    Retroviral

    Tags

    Mainly mouse Ap1g1 cDNA, with a fragment of brain…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA1_GAL4UAS-Luciferase reporter

    Plasmid

    #64157

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBstSK+IRES-V5-IPpoI

    Plasmid

    #206321

    Purpose

    A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

    Depositor

    Brian McStay

    Article

    van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

    Insert

    IRES element-V5 epitope tag- IPpoI open reading frame
    

    Use

    Mrna production

    Tags

    V5-IPpoI fusion protein

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgRNA2_GAL4UAS-Luciferase reporter

    Plasmid

    #64158

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA2 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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