2,226 results

1. pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3

   Plasmid

   #165430

   Purpose

   Expresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   FHS-paCaMKII(SD)
   

   Use

   AAV

   Tags

   FHS(Flag, HisX6, Strep) tag

   Expression

   Mammalian

   Mutation

   C450A, L514K, G528A, L531E, N538E: Light-insensit…

   Promoter

   CaMKII0.4

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-PRMT1

   Plasmid

   #34699

   Depositor

   Steven Clarke

   Article

   Gary et al J Biol Chem. 1996 May 24;271(21):12585-9

   Insert

   PRMT1 (Prmt1 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

3. CFAST-Halotag-ER

   Plasmid

   #214413

   Purpose

   ER localization of CFAST fused to Halotag-ER

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   rat cb5 ERM
   

   Use

   Tags

   CFAST-Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

   Plasmid

   #105323

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7c variant 1513
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Stx4-pmCitrine

   Plasmid

   #92422

   Purpose

   Expression of syntaxin-4 C-terminally conjugated to mCitrine.

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

   Insert

   Stx4 (Stx4 Rat)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-HA-Nrxn1α-LNS3

   Plasmid

   #128107

   Purpose

   Express HA tagged Nrxn1α-LNS3

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nrxn1a-LNS3 (Nrxn1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HA-Nrxn1α-LNS1

   Plasmid

   #128105

   Purpose

   Express HA tagged Nrxn1α-LNS1

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nrxn1a-LNS1 (Nrxn1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. rLAMP1-mCherry-EGFP-Tet-On

   Plasmid

   #109015

   Purpose

   Tet-On construct to induce expression of tandem eGFP-mCherry tagged mLAMP1 (lysosome marker)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   rLAMP1
   

   Use

   Lentiviral

   Tags

   mcherry-eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-Flex-Axon-GCaMP7b

   Plasmid

   #135419

   Purpose

   Can be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.

   Depositor

   Rylan Larsen

   Insert

   axon-jGCaMP7b
   

   Use

   AAV

   Tags

   6xHis and GAP43 palmitoylation domain

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CamKII-ADAM23-pHluorin

    Plasmid

    #185539

    Purpose

    Expresses ADAM23-pHluorin under the CamKII promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    ADAM23 (Adam23 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE GFP-CACNA1A KI

    Plasmid

    #131480

    Purpose

    Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacna1a, Cacna1a Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p130Cas Y15F

    Plasmid

    #186154

    Purpose

    For expressing recombinant p130Cas in insect cells.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    BCAR1 (Bcar1 Rat)
    

    Use

    Tags

    His6 (Tev)

    Expression

    Insect

    Mutation

    15Y residues mutated to Alanine

    Promoter

    polyhedrin

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-myc-ERK2-L4A-MEK1_fusion

    Plasmid

    #39197

    Depositor

    Melanie Cobb

    Article

    Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

    Insert

    ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    L4A = L33A, L37A, L40A, L42A

    Promoter

    CMV

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

    Plasmid

    #74309

    Purpose

    Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Human, Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-MPAct-mCit-IRES-Neo

    Plasmid

    #155230

    Purpose

    lentiviral MPAct construct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lentiviral expression of MPAct-mCitrine sensor
    

    Use

    Lentiviral

    Tags

    mCitrine

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBa.GFP-myosin Vb tail 1436-1846

    Plasmid

    #162714

    Purpose

    Mammalian expression of rat WT Myosin Vb with deletion of the 1435 N-terminal residues

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Myosin Vb (Myo5b Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 1435 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. RIP Cre-ER (DM#265)

    Plasmid

    #15029

    Depositor

    Douglas Melton

    Article

    Dor et al Nature. 2004 May 6. 429(6987):41-6.

    Insert

    Rat insulin promoter (RIP) (Ins2 Rat)
    

    Use

    Cre/Lox; For making transgenic constructs

    Tags

    Cre-ER (TM)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-2dV-Camui (T305D/T306D)

    Plasmid

    #220368

    Purpose

    T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    

    Use

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Mutation

    changed both Threonine 305 and 306 to aspartic ac…

    Promoter

    CAG

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCer-4aa-VAMP2

    Plasmid

    #53233

    Purpose

    Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1

    Plasmid

    #165444

    Purpose

    Expresses ACX, rXRCC1 in mammalian cells

    Depositor

    Wei Leong Chew

    Article

    Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

    Insert

    APOBEC-nCas9-rXRCC1 (Apobec1 Rat, Synthetic, Streptococcus pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge