Skip to main content
Addgene
Showing: 1081 - 1120 of 3893 results

  1. pFrt-invCAG-Luc

    Plasmid
    #63577
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sites
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63577-plasmid-map-sequence-id-105728

    Enlarge

  10. pCC324

    Plasmid
    #110165
    Purpose
    miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    R61K change made in ERK-KTR CDS
    Promoter
    lin-31 promoter/enhancer
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110165-plasmid-map-sequence-id-235338

    Enlarge

  11. p405CYC1

    Plasmid
    #15967
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    p405CYC1 (CYC1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    CYC1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15967-plasmid-map-sequence-id-8452

    Enlarge

  13. pC28-LIC-His

    Plasmid
    #182504
    Purpose
    pET based vector for protein expression in E.coli for targets fused with C-terminal His
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Use
    Tags
    TEV-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182504-plasmid-map-sequence-id-358072

    Enlarge

  20. pLXSN p110 CUX1

    Plasmid
    #90471
    Purpose
    Retroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectively
    Depositor
    Alain Nepveu
    Article
    Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11
    Insert
    CUX1 (amino acids 747-1505) (CUX1 Human)
    Use
    Retroviral
    Tags
    HA and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Moloney murine leukemia virus long terminal repeat
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    90471-plasmid-map-sequence-id-205963

    Enlarge

  24. EC71139

    Plasmid
    #154062
    Purpose
    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    pZmUbi
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    All BsaI, Esp3I and BpiI restriction sites were r…
    Promoter
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154062-plasmid-map-sequence-id-312952

    Enlarge

  31. pEP4 E02S EM2K

    Plasmid
    #20923
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4
    Depositor
    James Thomson
    Article
    Yu et al Science. 2009 Mar 26.
    Insert
    Oct4 and Sox2; Myc and Klf4 (SOX2, KLF4, MYC, POU5F1 Human)
    Use
    Episomal/ebna
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    20923-plasmid-map-sequence-id-272720

    Enlarge

  32. pXJ42-p110 CUX1

    Plasmid
    #100814
    Purpose
    Plasmid vector expressing human p110 CUX1 (amino acids 878-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag.
    Depositor
    Alain Nepveu
    Article
    Truscott et al Mol Cell Biol. 2008 May;28(10):3127-38.
    Insert
    p110 CUX1 (amino acids 878-1505) (CUX1 Human)
    Use
    Tags
    HA and Myc
    Expression
    Mammalian
    Mutation
    Contains amino acids 878-1505
    Promoter
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    100814-plasmid-map-sequence-id-205853

    Enlarge

  33. pLenti6/V5-DEST-p110 CUX1

    Plasmid
    #100816
    Purpose
    Gateway lentiviral vector expressing p100 CUX1 (amino acids 747-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag
    Depositor
    Alain Nepveu
    Article
    Vadnais et al BMC Genomics. 2013 Apr 16;14:258. doi: 1
    Insert
    Hs CUX1 amino acids 747-1505 of P39880 (CUX1 Human)
    Use
    Lentiviral
    Tags
    HA and myc
    Expression
    Mammalian
    Mutation
    contains amino acids 747-1505
    Promoter
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    100816-plasmid-map-sequence-id-189653

    Enlarge

Showing: 1081 - 1120 of 3893 results