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pSG 163

Plasmid

#63871

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAG near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pDL11

Plasmid

#17832

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, argB,

Use

Synthetic Biology; Aspergillus nidulans expression

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pGGEV_5'_Linker

Plasmid

#49291

Purpose

Golden Gate entry vector at position 5, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_+1_Linker

Plasmid

#49280

Purpose

Golden Gate entry vector at position 1,small linker insert, plus 5' ATG

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCM5.31

Plasmid

#19398

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

par-5 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pICH41295(pro5U)-pAT2G29080(D,C)

Plasmid

#205370

Purpose

MoClo lvl0 Promoter + 5'UTR part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

pAT2G29080(D,C)

Use

Synthetic Biology

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41295(pro5U)-pAT3G08530(H,I)

Plasmid

#205375

Purpose

MoClo lvl0 Promoter + 5'UTR part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

pAT3G08530(H,I)

Use

Synthetic Biology

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41295(pro5U)-pAT5G37830(B,E)

Plasmid

#205384

Purpose

MoClo lvl0 Promoter + 5'UTR part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

pAT5G37830(B,E)

Use

Synthetic Biology

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41295(pro5U)-pAT1G64550(E,A)

Plasmid

#205363

Purpose

MoClo lvl0 Promoter + 5'UTR part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

pAT1G64550(E,A)

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41295(pro5U)-pAT2G26780(A,B)

Plasmid

#205368

Purpose

MoClo lvl0 Promoter + 5'UTR part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

pAT2G26780(A,B)

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41295(pro5U)-pAT3G18480(F,G)

Plasmid

#205377

Purpose

MoClo lvl0 Promoter + 5'UTR part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

pAT3G18480(F,G)

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGGEV_5'_XcmI-LacZ

Plasmid

#49306

Purpose

Golden Gate entry vector at position 5, LacZ, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_+1_XcmI-LacZ

Plasmid

#49295

Purpose

Golden Gate entry vector at position 1, plus 5' ATG, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177)

Plasmid

#225442

Purpose

BeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

LIR:2nd intron:3'eGFP:T35S:SIR:p35S:5'eGFP:1st intron:LIR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHisAlix

Plasmid

#42576

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

ALIX (PDCD6IP Human)

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pDY1173_ADAR1p150

Plasmid

#193192

Purpose

Express full length ADAR1p150

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

ADAR1p150 (ADAR Human)

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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HP-Cas9hGem-Ade2-LEU2-LINEAR

Plasmid

#174839

Purpose

pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha

Use

CRISPR and Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TDH3p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET-TRSter

Plasmid

#78865

Purpose

Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

Depositor

Elias Arnér

Article

Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

Insert

Thioredoxin Reductase (Txnrd1 Rat)

Tags

Mutated E. coli-type SECIS element (3’ end of ins…

Expression

Bacterial

Promoter

T7lac

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK3 S368A

Plasmid

#122680

Purpose

Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Expression

Mammalian

Mutation

changed Serine 368 to Alanine

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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T7-p53DD-pcDNA3

Plasmid

#25989

Depositor

William Kaelin

Article

Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

Insert

tumor protein p53 (TP53 Human)

Tags

T7

Expression

Mammalian

Mutation

truncated cDNA encodes truncated protein (aa 300-…

Available Since

Oct. 21, 2010

Availability

Academic Institutions and Nonprofits only

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