Addgene

Showing: 541 - 560 of 1919 results

Results per page:

Sort results by:

FUW-Flag-Mbd3 delta1-70

Plasmid

#52372

Purpose

Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

deleted amino acids 1-70

Promoter

Ubiquitin

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-Flag-Mbd3 delta 25-83

Plasmid

#52373

Purpose

Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

amino acids 25-83 deleted

Promoter

Ubiquitin

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-Flag-Mbd3 delta49-63

Plasmid

#52374

Purpose

Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

deleted amino acids 49-63

Promoter

Ubiquitin

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRPy CAGGS-Mbd3-IRES-PURO

Plasmid

#52376

Purpose

Mammalian expression vector for constitutive expression of Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Tags

2xFlag (DYKDDK)

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-human OCT4 PE-SV40-Luc

Plasmid

#52415

Purpose

Luciferase reporter plasmid for PE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Proximal element of hOCT4 promoter (POU5F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

Plasmid

#52419

Purpose

Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

ERAS (ERAS Human)

Use

Tags

Expression

Mutation

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-human OCT4 DE-SV40-Luc

Plasmid

#52414

Purpose

Luciferase reporter plasmid for DE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Distal element of human OCT4 promoter (POU5F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_CFLAR_WT_V5

Plasmid

#82936

Purpose

Gateway Donor vector containing CFLAR, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Matthew Meyerson, Jesse Boehm, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

CFLAR (CFLAR Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP11633 - pAAV-hsA2-AiE0140h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1633)

Plasmid

#163503

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Mich et al Cell Rep. 2021 Mar 30;34(13):108754. doi

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

MinRho

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CN1457-rAAV-eHGT_078h-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#163495

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Mich et al Cell Rep. 2021 Mar 30;34(13):108754. doi

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBetaGlobin

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP11402-pAAV-AiE0058h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1402)

Plasmid

#163494

Purpose

AiE0058h is an enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Mich et al Cell Rep. 2021 Mar 30;34(13):108754. doi

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBetaGlobin

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CN1621-rAAV-hsA2-eHGT_128h-minRho-SYFP2-WPRE3-BGHpA

Plasmid

#163498

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Mich et al Cell Rep. 2021 Mar 30;34(13):108754. doi

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

MinRho

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
delta PE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52380

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted -1620 - -1011 upstream from OCT4 ATG

Promoter

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
CN1528-rAAV-hsA2-eHGT_082h-minRho-SYFP2-WPRE3-BGHpA

Plasmid

#163502

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Mich et al Cell Rep. 2021 Mar 30;34(13):108754. doi

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

MinRho

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
delta DE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52381

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted -3194 - -1768 upstream from OCT4 ATG

Promoter

OCT4

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
CN1253-scAAV-eHGT_017h-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#163497

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Mich et al Cell Rep. 2021 Mar 30;34(13):108754. doi

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBetaGlobin

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mouse deltaPE Oct4-GFP transgenic reporter

Plasmid

#52382

Purpose

BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP pA
Oct4 (Pou5f1 Mouse)
PGK-gb2-Neo

Use

Tags

Expression

Mammalian

Mutation

deleted -300 - -1300bp fragment upstream from Oct…

Promoter

Oct4 and PGK+gb2

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP11408-pAAV-AiE0064h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1408)

Plasmid

#163500

Purpose

AiE0064h is an enhancer sequence designed to drive AAV-mediated transgene expression in Pvalb+ and Sst+ cortical interneurons

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Mich et al Cell Rep. 2021 Mar 30;34(13):108754. doi

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBetaGlobin

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CN1525-rAAV-hsA2-eHGT_079h-minRho-SYFP2-WPRE3-BGHpA

Plasmid

#163501

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Mich et al Cell Rep. 2021 Mar 30;34(13):108754. doi

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

MinRho

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12045-pAAV-3xSP10ins-AiE0359h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2045)

Plasmid

#163504

Purpose

AiE0359h is an enhancer sequence designed to drive AAV-mediated transgene expression in Pvalb+ cortical interneurons and cerebellar Purkinje cells

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Mich et al Cell Rep. 2021 Mar 30;34(13):108754. doi

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

MinRho

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge