We narrowed to 3,282 results for: cgas

1. pJRH029

   Plasmid

   #171060

   Purpose

   Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   Gag-spyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x SV40 NLS and 3x FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDECKO_GFP

   Plasmid

   #72619

   Purpose

   Expresses two gRNAs targeting GFP

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAT009

   Plasmid

   #171635

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRH-1346 U6-B2M sgRNA Gag-pol v2

   Plasmid

   #201917

   Purpose

   Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-pol
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and Human U6

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5FRT-EF-Pdgfralpha-EGFPN

   Plasmid

   #66787

   Purpose

   Expression of murine PDGFRalpha tagged with GFP under the control of EF1a promoter

   Depositor

   Lotte Pedersen

   Article

   Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

   Insert

   PDGFRalpha (Pdgfra Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #176272

   Purpose

   Expression of non-targeting sgRNA of CasMINI

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI non-targeting sgRNA (sgLacZ) and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. L4440_BioBrick-SCR-sgRNA-A

   Plasmid

   #177811

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX304-mNeonGreen

   Plasmid

   #162034

   Purpose

   Tagged vector control

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Neon Green

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPRv2-Beclin1

   Plasmid

   #99574

   Purpose

   Expressed Cas9 with sgRNA targeting Beclin1

   Depositor

   Edward Campbell

   Article

   Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

   Insert

   gRNA targeting Beclin1 (BECN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJR022

    Plasmid

    #198820

    Purpose

    C. elegans optimized version of mScarlet

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::wrmScarlet::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAT005

    Plasmid

    #171632

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. NK73

    Plasmid

    #176609

    Purpose

    To test bead engulfment of macrophages.

    Depositor

    Meghan Morrissey

    Article

    Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

    Insert

    SNAP-CD86-FCERG (CD86 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pARF6(T27N)-CFP

    Plasmid

    #11386

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 6 (ARF6 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 27 to Asparagine.

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pARF6(Q67L)-CFP

    Plasmid

    #11387

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 6 (ARF6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHW450

    Plasmid

    #198831

    Purpose

    Voltage-sensitive fluorescent protein.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAT007

    Plasmid

    #171634

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJRH623

    Plasmid

    #171633

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-non-targeting
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)

    Plasmid

    #179004

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBB75-ncRNA

    Plasmid

    #194087

    Purpose

    Expresses Ec86 ncRNA in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 ncRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLEX301-TagRFP

    Plasmid

    #162035

    Purpose

    Tagged vector control

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    RFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge