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pcDNA3-HA-eEF2K

Plasmid

#110160

Purpose

Expression of human eEF2K in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

Insert

eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRPM5

Plasmid

#85189

Purpose

Expresses full-length mouse TRPM5 in mammalian cells

Depositor

Craig Montell

Article

Hofmann et al Curr Biol. 2003 Jul 1;13(13):1153-8.

Insert

TRPM5 (Trpm5 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PPARgamma2

Plasmid

#78768

Purpose

To overexpress PPARgamma2 in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPARG (Pparg Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
T7-p53DD-pcDNA3

Plasmid

#25989

Depositor

William Kaelin

Article

Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

Insert

tumor protein p53 (TP53 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

truncated cDNA encodes truncated protein (aa 300-…

Promoter

Available since

Oct. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPR-A

Plasmid

#89119

Purpose

mammalian expression of full-length human progesterone receptor-A, equivalent to human PR-B amino acids 165-933

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-A (PGR Human)

Use

Tags

Expression

Mammalian

Mutation

human progesterone receptor-A, equivalent to huma…

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-mRipk3

Plasmid

#78805

Purpose

To overexpress mRipk3 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (Ripk3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_CEACAM6-His

Plasmid

#149337

Purpose

for eukaryotic expression and purification of CEACAM6-His6

Depositor

James Fleckenstein

Article

Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

Insert

CEACAM6 (CEACAM6 Human)

Use

Tags

IL-2 signal peptide and polyhistidine

Expression

Mammalian

Mutation

point mutation (GGA to GCA) to replace 'G-g…

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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GATA3-pCDNA3.1

Plasmid

#115271

Purpose

Expresses human GATA3 in mammalian cells

Depositor

Arne S. Schaefer

Insert

GATA3 (GATA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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Pyronic/pcDNA3.1(-)

Plasmid

#51308

Purpose

Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

Insert

Pyronic

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mBeRFP

Plasmid

#175173

Purpose

large-stokes shift red fluorescent protein mBeRFP

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

mBeRFP

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hfYFP

Plasmid

#186523

Purpose

Hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Use

Tags

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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B-gTEMP/pcDNA3

Plasmid

#188447

Purpose

B-gTEMP for mammalian expression

Depositor

Takeharu Nagai

Article

Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

Insert

B-gTEMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNoxo1

Plasmid

#58338

Purpose

expresses mouse Noxo1 in mammalian cells

Depositor

Karl-Heinz Krause, Botond Banfi

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

NADPH oxidase organizer 1 (Noxo1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MEF2C-HA

Plasmid

#32515

Depositor

Andrew Lassar

Article

Kozhemyakina et al Mol Cell Biol. 2009 Nov;29(21):5751-62.

Insert

Mef2c (Mef2c Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNAFLAG-ATM-kd

Plasmid

#43908

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

ataxia telangiectasia mutated (ATM Human)

Use

Tags

FLAG and His

Expression

Mammalian

Mutation

kinase dead (D2870A and N2875K)

Promoter

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ar1

Plasmid

#225219

Purpose

Expression androgen receptor 1 in mammalian cells

Depositor

Deshou Wang

Article

Xiao et al Commun Biol. 2024 Sep 16;7(1):1154. doi:

Insert

androgen receptor 1

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-H-Luc_cyto

Plasmid

#113929

Purpose

Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/cmycDrosha

Plasmid

#10828

Depositor

Thomas Tuschl

Article

Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

Insert

Drosha (DROSHA Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3 hE47

Plasmid

#16059

Depositor

Robert Benezra

Article

Jen et al Genes Dev. 1992 Aug . 6(8):1466-79.

Insert

E47 (TCF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TYMS

Plasmid

#100545

Purpose

mammalian expression of thymidylate synthetase

Depositor

Yuh-Shan Jou

Article

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

thymidylate synthetase (TYMS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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