We narrowed to 1,093 results for: pcs2

1. GSK-3beta-his (Xenopus)/pCS2+

   Plasmid

   #16335

   Depositor

   Peter Klein

   Insert

   glycogen synthase kinase-3beta (gsk3b.L Frog)
   

   Use

   Vertebrate expression

   Tags

   6xHis

   Expression

   Mutation

   Xenopus GSK-3 with 6X his tag

   Promoter

   Available Since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pCS2 Flag Smad3 EPSM A204S

   Plasmid

   #27111

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Use

   Xenopus/mammalian/avian/zebrafish

   Tags

   Flag

   Expression

   Mutation

   T179V S204A S208A S213A; A204S

   Promoter

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pCS2-MYC-GFP-IRES-mCherry

   Plasmid

   #231232

   Purpose

   MYC stability reporter construct (expressing c-myc 2) for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-MCRS1-GFP-IRES-mCherry

   Plasmid

   #231231

   Purpose

   MCRS1 stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MCRS1 (MCRS1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcs2-cDELE1(142-515)-sumo

   Plasmid

   #226107

   Purpose

   cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   DELE1 (DELE1 Human)
   

   Use

   Tags

   Sumo

   Expression

   Mammalian

   Mutation

   encodes only residues 142-515 of DELE1 (represent…

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-HsProm1s1(W795R)-TwinStrep-His

   Plasmid

   #210817

   Purpose

   Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (W795R)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Mammalian

   Mutation

   Changed Trp-795 to Arg

   Promoter

   CMV

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-foxd4l1.1-deltaRII-Cterm-myc

   Plasmid

   #185505

   Purpose

   Expression of Xenopus laevis foxd4

   Depositor

   Sally Moody

   Article

   Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

   Insert

   foxd4l1.1
   

   Use

   In vitro transcription

   Tags

   myc

   Expression

   Mutation

   RII domain and C-term deleted

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcs2-MTS-cox8A-GFP-IRES-mCherry

   Plasmid

   #226103

   Purpose

   Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   COX8A (COX8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   encodes only for mitochondrial targeting sequence…

   Promoter

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)

   Plasmid

   #156407

   Purpose

   pCS2 CMV expression vector driving EGFP fused human KRAS4B-G12V

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4B-G12V (KRAS Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   mutant KRAS4B-G12V

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2 LRP6-APEX2-Ires-eGFP-PAC

    Plasmid

    #180142

    Purpose

    used to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods

    Depositor

    Edward De Robertis

    Article

    Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

    Insert

    Human LRP6 (LRP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2 stabilized mutant Beta Catenin-GFP

    Plasmid

    #29684

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    beta catenin (ctnnb1.L Frog)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S33A, S37A, T41A

    Promoter

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

    Plasmid

    #154910

    Purpose

    1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.

    Depositor

    Jonathan Clarke, Clare Buckley

    Article

    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

    Insert

    Phytochrome B (PHYB Mustard Weed, Synthetic)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    CAAX membrane moiety and mCherry

    Expression

    Mammalian

    Mutation

    1-621 amino acids of Arabidopsis PHYB

    Promoter

    CMV

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+ Gal4-GBP2-IRES-GBP7-p65

    Plasmid

    #50020

    Purpose

    Multi-purpose expression of a GFP-dependent transcription factor

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD
    

    Use

    Multi-purpose expression

    Tags

    Expression

    Mutation

    Promoter

    CMV-immediate early promoter and SP6 promoter

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)

    Plasmid

    #156410

    Purpose

    pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12V

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12V (KRAS Human)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    mutant KRAS4A-G12V

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)

    Plasmid

    #156409

    Purpose

    pCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12D

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    mutant KRAS4A-G12D

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2plus-mCerulean 156-239-GGGS-TDP-43

    Plasmid

    #162617

    Purpose

    Allows for transcription of mCerulean fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2plus-mVenus1-155-GGGS-TDP43 M337V

    Plasmid

    #162612

    Purpose

    Allows for transcription of mVenus fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M337V

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2plus-mVenus1-155-I152L-GGGS-TDP-43

    Plasmid

    #162614

    Purpose

    Allows for transcription of improved mVenus I152L fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2-Sun1-2xsfGFP-6xMYC-pA (JDW 694)

    Plasmid

    #242588

    Purpose

    A CMV driven expression vector containing a Sun1-2xsfGFP for labeling nuclear envenlope.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Sun1-2xsfGFP-6xMYC (Sun1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+8-CYP2A6(C82A)-flag-tev-his

    Plasmid

    #227166

    Purpose

    For overexpression of CYP2A6 C82A mutant in mammalian cells and purification.

    Depositor

    Yimon Aye

    Article

    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

    Insert

    CYP2A6 (CYP2A6 Human)
    

    Use

    Tags

    Flag and His6

    Expression

    Mammalian

    Mutation

    changed cysteine 82 to alanine

    Promoter

    CMV and SP6

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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