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pHCMV_YFV_PrME

Plasmid

#207315

Purpose

Mammalian expression of Yellow fever virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

YFV_PrME (POLY Yellow fever virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Lv EF1a MS2-Fkbp2x 2A Hygro

Plasmid

#102807

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

MS2-Fkbp2x

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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MKbpAII

Plasmid

#91689

Purpose

MMTV-LTR promoter/enhancer with rabbit beta-globin intron and bovine growth hormone poly-A sequence to enhance transgene expression

Depositor

Jeffrey Rosen

Article

Tepera et al J Cell Sci. 2003 Mar 15;116(Pt 6):1137-4

Insert

MMTV LTR cloned into KbpA vector

Expression

Mammalian

Promoter

MMTV LTR

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDESTmycPABPC4

Plasmid

#19877

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

PABPC4 (PABPC4 Human)

Tags

MYC

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb59H10

Plasmid

#184682

Purpose

NIR-Fb to HIV p24

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb59H10

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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R26TV CAG LSL 2-5

Plasmid

#68412

Purpose

Rosa26 targeting vector backbone for use with GMAP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting; Gmap

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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MET-HYGRO

Plasmid

#218113

Purpose

Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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LYS-Leu2

Plasmid

#218114

Purpose

Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV_MARV_GP

Plasmid

#207292

Purpose

Mammalian expression of Lake victoria Marburgvirus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

MARV_GP (GP Lake victoria Marburgvirus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBS Olfactomedin1

Plasmid

#19225

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Olfactomedin 1 (OLFM1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pEX-A-U6-gRNA

Plasmid

#65626

Purpose

Empty vector for the expression U6 driven gRNA

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

U6-gRNA

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pET303-colE1T

Plasmid

#155180

Purpose

Expresses colicin E1 residues 1-190

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

colicin E1 residues 1-190

Tags

C-terminal 6x Histidine tag

Expression

Bacterial

Mutation

truncation of colicin E1 including 1-190

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHCMV_TBEV_PrME

Plasmid

#207310

Purpose

Mammalian expression of Tick-borne encephalitis virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

TBEV_PrME (POLY Tick-borne encephalitis virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hRabin8 WT

Plasmid

#26726

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pDYU1B/mRuby3–α-tubulin

Plasmid

#154302

Purpose

Expresses mRuby3–α-tubulin from actin15 promoter in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

alpha-tubuin

Use

Dictyostelium expression

Tags

mRuby3

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-2A-TurboGFP-PEST-PuroR

Plasmid

#80956

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TurboGFP-PEST

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PM-Ras-LOCKR-S

Plasmid

#216498

Purpose

FRET-based reporter for Ras activity, plasma membrane localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

PM-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pT2HLacZpA1I.7 (#59)

Plasmid

#35965

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Inserts

Tie2 promoter (Tek Mouse)
SDK LacZ
SV40 pA
Tie2 full enhancer

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pWS5321-CRISPRai-Markerless

Plasmid

#182828

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - markerless

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBac-DsRed-ORCO_9kbProm-QF2

Plasmid

#104877

Purpose

Anopheles gambiae ORCO enhancer/promter to drive QF2 expression in Anopheles transgenic mosquitoes

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Insert

AgORCO-QF2

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y397F

Plasmid

#50503

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y397F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
Integrin-β6 I domain I270C

Plasmid

#159639

Purpose

Contains integrin beta 6 residues 108–352 with I270C mutation

Depositor

Timothy Springer

Article

Dong et al Nature. 2017 Feb 2;542(7639):55-59. doi:

Insert

Integrin beta 6 I domain I270C

Tags

6x His

Expression

Mammalian

Mutation

I270C

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTmpSm

Plasmid

#176228

Purpose

A template plasmid for amplification of the cI-hok-strA cassette

Depositor

Sergey Sineoky

Article

Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

Insert

cI of phage lambda, hok of the R1 plasmid, strA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMU-MITr

Plasmid

#61198

Purpose

Fluorescent reporter for mitochondria ScCOX4sp-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

ScCOX4sp-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV_EIAV_Env

Plasmid

#207326

Purpose

Expression of env polyprotein from equine infectious anaemia (EIAV)

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

EIAV_Env

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pActP-dtrpA1

Plasmid

#64713

Purpose

inducing calcium influx in cultured Drosophila S2 cells

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

dtrpA1 (TrpA1 )

Expression

Insect

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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CAG-mBrn2

Plasmid

#19711

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

mouse Brn2 (Pou3f2 Mouse)

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-ATP

Plasmid

#184599

Purpose

For expression of a fluorescent sensor for ATP in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-ATP

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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SaABEmax

Plasmid

#119814

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSaCas9

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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ER-moxMaple3

Plasmid

#120885

Purpose

mammalian expression of ER localized moxMaple3

Depositor

Erik Snapp

Article

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Insert

moxMaple3

Tags

KDEL ER retention sequence and bovine prolactin s…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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