We narrowed to 10,796 results for: HA

1. pRQ-hRasV12

   Plasmid

   #18113

   Depositor

   Alex Swarbrick

   Article

   Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 10

   Insert

   h-RasV12G (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V, constitutively active

   Promoter

   Available since

   Feb. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. TJP1_PDZ_1

   Plasmid

   #103909

   Purpose

   Protein expression and purification of ZO1 PDZ1 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   TJP1 (TJP1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis(TEV)-dDrp1-StrepII

   Plasmid

   #174421

   Purpose

   Expresses D. melanogaster Drp1 in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1 (Drp1 Fly)
   

   Use

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKnockinDonor-humanCTCF-miniAID-mClover3

   Plasmid

   #136883

   Purpose

   knockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCF

   Depositor

   Chunliang Li

   Article

   Hyle et al Nucleic Acids Res. 2019 Jul 26;47(13):66

   Insert

   CTCF-knockin-homology arm-miniAID-mClover3
   

   Use

   Tags

   miniAID-mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-CHRFAM7A-mGFP

   Plasmid

   #62633

   Purpose

   mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loop

   Depositor

   Henry Lester

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   CHRFAM7A (CHRFAM7A Human)
   

   Use

   Tags

   mEGFP inserted in M3-M4 loop

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC147-pCR8-dCas9VP64

   Plasmid

   #48219

   Purpose

   dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP64 activation domain
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag and VP64

   Expression

   Mutation

   D10A;H840A

   Promoter

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLXSN-H-Ras_V12

   Plasmid

   #39516

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   HRAS V12 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pET28-His8-aCat(660-906)

   Plasmid

   #203333

   Purpose

   Bacterial expression of alpha-catenin F-actin binding domain

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Use

   Tags

   8xHis with precision protease cleavage site

   Expression

   Bacterial

   Mutation

   aa 660-906 only

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TIAM1_PDZ_1

   Plasmid

   #103925

   Purpose

   Protein expression and purification of TIAM PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMT-ubb-cytoBirA-2a-mCherry

    Plasmid

    #79885

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80058

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-mCherry-p2A-LIC1 Y101A, Y103A

    Plasmid

    #74605

    Purpose

    expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Y101A, Y103A

    Promoter

    SFFV

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST40-2XHA-Src-K298M-Y419F-Y530F

    Plasmid

    #140320

    Purpose

    Expresses HA-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    K298M-Y419F-Y530F

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRE-UBC-Crry-T2A-GFP

    Plasmid

    #205452

    Purpose

    lentiviral plasmid for expression of mouse Crry

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    Crry (Cr1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PDLIM7_PDZ_1

    Plasmid

    #103940

    Purpose

    Protein expression and purification of ENIGMA PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDLIM7 (PDLIM7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. UB061 (FCK-stGtACR2-fusionRed)

    Plasmid

    #135765

    Purpose

    expresses soma targeted GtACR2

    Depositor

    Adam Cohen

    Article

    Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

    Insert

    stGtACR2
    

    Use

    Lentiviral

    Tags

    Fusion Red

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST40-2XHA-Src-KD

    Plasmid

    #140312

    Purpose

    Expresses HA-tagged human Src-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Contains only the KD region

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS-AMV-α1L9'S-nAchR

    Plasmid

    #20684

    Depositor

    Henry Lester

    Article

    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

    Insert

    nicotinic acetylcholine receptors (Chrna1 Mouse)
    

    Use

    Tags

    AMV and pA 50

    Expression

    Bacterial

    Mutation

    Leucine at 9' position in M2 region changed …

    Promoter

    Available since

    April 3, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pBabe puro H-Ras 12V (37G)

    Plasmid

    #12589

    Depositor

    Channing Der

    Article

    McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

    Insert

    HRas 12V 37G (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    12V, 37G

    Promoter

    Available since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pCMV5 Flag Jnk1a1(APY)

    Plasmid

    #15742

    Depositor

    Roger Davis

    Article

    Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

    Insert

    Jnk1a1(TPF) (MAPK8 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Thr-190Ala

    Promoter

    Available since

    March 6, 2008

    Availability

    Academic Institutions and Nonprofits only