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pAAV-nEF-SpCas9D10A nickase

Plasmid

#216736

Purpose

Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

Depositor

Vincent Dion

Article

Alvaro et al bioRxiv

Insert

Cas9-D10A nickase

Use

AAV and CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

EF-1-alpha core

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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LV-PGK-PVR-P2A-Cre-EFS-mScarletSIIN

Plasmid

#172435

Purpose

Lentivirus, expresses Cre recombinase, mouse PVR (CD155), and mScarlet-SIINFEKL

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
PVR
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

Lentiviral

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_Deletion_Upstream_gRNA_2

Plasmid

#195135

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Upstream gRNA 2 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_Deletion_Upstream_gRNA_1

Plasmid

#195134

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Upstream gRNA 1 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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R620-M18-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6 K417T/E484K/N501Y

Plasmid

#170202

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

K417T, E484K, N501Y

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M19-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417T/E484K/N501Y

Plasmid

#170203

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

3C-His8-SBP

Expression

Mammalian

Mutation

K417T, E484K, N501Y

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

Plasmid

#170191

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

K417N, E484K, N501Y

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAB(EXPR-DS-HDAC5_HDAC3)

Plasmid

#114399

Purpose

For PYL-HDAC5 catalytic domain swap to HDAC3 catalytic domain expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5_HDAC3 catalytic domain swap (HDAC3, HDAC5 Human)

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXI3

Plasmid

#101518

Purpose

Donor Vector containing FOXI3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXI3 (FOXI3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ASCL3

Plasmid

#101515

Purpose

Donor Vector containing ASCL3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ASCL3 (ASCL3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-HOXB3

Plasmid

#101478

Purpose

Donor Vector containing HOXB3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HOXB3 (HOXB3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXE1

Plasmid

#101439

Purpose

Donor Vector containing FOXE1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXE1 (FOXE1 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXE3

Plasmid

#101429

Purpose

Donor Vector containing FOXE3 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXE3 (FOXE3 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXH1

Plasmid

#101431

Purpose

Donor Vector containing FOXH1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXH1 (FOXH1 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-BSX

Plasmid

#101410

Purpose

Donor Vector containing BSX transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

BSX (BSX Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-HOXB9

Plasmid

#101398

Purpose

Donor Vector containing HOXB9 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HOXB9 (HOXB9 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-HOXC5

Plasmid

#101399

Purpose

Donor Vector containing HOXC5 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HOXC5 (HOXC5 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-HOXD8

Plasmid

#101402

Purpose

Donor Vector containing HOXD8 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

HOXD8 (HOXD8 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ETV3

Plasmid

#88804

Purpose

Donor Vector containing ETV3 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ETV3 (ETV3 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4(TA)-ires-blast

Plasmid

#167831

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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