We narrowed to 31,511 results for: REP

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pCSIIpuro-EGF-ScNeo

Plasmid

#209893

Purpose

To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

EGF-ScNeo (EGF Human)

Use

Lentiviral

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-HBEGF-ScNeo

Plasmid

#209894

Purpose

To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

HBEGF-ScNeo (HBEGF Human)

Use

Lentiviral

Tags

mNeonGreen and mScarlet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-Blast FAP S624A

Plasmid

#207402

Purpose

Expresses the S624A mutant of FAP (Fibroblast Activation Protein), which shows no protease activity.

Depositor

Jing Yang

Article

Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

Insert

Fibroblast Activation Protein Alpha (FAP Human)

Use

Retroviral

Mutation

changed Serine 624 to Alanine. It's a protea…

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL-Blast FAP A657S

Plasmid

#207403

Purpose

Expresses the A657S mutant of FAP (Fibroblast Activation Protein), which shows partial protease activity.

Depositor

Jing Yang

Article

Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

Insert

Fibroblast Activation Protein Alpha (FAP Human)

Use

Retroviral

Mutation

changed Alanine 657 to Serine. It's an endop…

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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VGlut2 scFv [N29/29]

Plasmid

#182085

Purpose

Mammalian Expression of VGlut2 scFv. Derived from hybridoma N29/29.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting VGlut2 (Rattus norvegicus) (Slc17a6 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pelB-MBP-HtrA-ss

Plasmid

#137066

Purpose

E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66500.2 (BB_0104 Borrelia burgdorferi B31)

Tags

PelB signal peptide + His10 + maltose binding pro…

Expression

Bacterial

Mutation

mature HtrA: lacks the HtrA signal peptide

Promoter

T7lac

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_1_001

Plasmid

#167347

Purpose

ddPCR gating control for droplets containing all 5 targets (2 in pol)

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat_env

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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PB-PEmax NG

Plasmid

#187894

Purpose

All-in-one prime editor piggyBac transposon with PEmax, NG variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Tom20-CR

Plasmid

#171461

Purpose

Expresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

Tom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

Plasmid

#139089

Purpose

Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

xCas9-NG

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC1 [1F1R]

Plasmid

#177437

Purpose

Mammalian Expression Plasmid of anti-TrpC1 (Human). Derived from hybridoma 1F1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpC1 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Pancortin scFv [K96/7]

Plasmid

#190497

Purpose

Mammalian Expression of Pancortin scFV. Derived from hybridoma K96/7.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pancortin (Homo sapiens) recombinant scFV (OLFM1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_9_003

Plasmid

#167349

Purpose

ddPCR gating control for droplets containing either gag+env targets OR tat + env targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gag_tat_env

Use

Other

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_6_002

Plasmid

#167348

Purpose

ddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat

Use

Other

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_29_006

Plasmid

#167352

Purpose

ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_env

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_14_004

Plasmid

#167350

Purpose

ddPCR gating control for droplets containing the env target

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

env

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Seattle_IPDA_control_32_007

Plasmid

#167353

Purpose

ddPCR gating control for droplets containing 5'pol or 3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_17_005

Plasmid

#167351

Purpose

ddPCR gating control for droplets containing gat and tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gat_tat

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Tom20-mChF-AIP

Plasmid

#61512

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP, Tom20, and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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AAV8-hSyn-flex-miR30-eGFP-shDyn

Plasmid

#132716

Purpose

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Pdyn gene, which encodes dynorphin

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

GFP-shDyn (Pdyn Rat)

Use

AAV

Promoter

Synapsin 1

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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