We narrowed to 10,134 results for: ada

1. pET48 AtGUN1-PPR2

   Plasmid

   #136360

   Purpose

   Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

   Depositor

   Tatsuru Masuda

   Article

   Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

   Insert

   cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)
   

   Tags

   TRX-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDB2-hPER1-mScarlet-I-CD4-bla

   Plasmid

   #189980

   Purpose

   Donor Vector to integrate mScarlet-I C-terminal into the human PER1 Locus to express it as a fusion protein

   Depositor

   Achim Kramer

   Article

   Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

   Insert

   mScarlet-I
   

   Use

   CRISPR

   Tags

   3xFlag

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5FRT/TO-PNGase-Strep-HA

   Plasmid

   #113490

   Purpose

   Expression of human PNGase with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   PNGase (NGLY1 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 βarr1 S412A Flag

   Plasmid

   #42195

   Depositor

   Robert Lefkowitz

   Article

   Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

   Insert

   β-arrestin 1 S412A (Arrb1 Rat)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S412A

   Promoter

   CMV

   Available Since

   Jan. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1P POLD1_2

   Plasmid

   #160792

   Purpose

   Suppress POLD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shPOLD1_2
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRL838

   Plasmid

   #70680

   Purpose

   This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

   Depositor

   Peter Wolk

   Article

   Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53

   Type

   Empty backbone

   Use

   Cre/Lox

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N2-RIPK1 3KR

   Plasmid

   #78847

   Purpose

   To detect localization of RIPK1 3KR protein in live mammalian cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K571R, K604R, K627R

   Promoter

   CMV

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3 myc card9 mutant

   Plasmid

   #16254

   Depositor

   William Kaelin

   Article

   Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

   Insert

   card9 mutant (CARD9 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S528A, T531A, T533A, S536A

   Available Since

   Nov. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-AMPKsub(TA)-YPet-NES

   Plasmid

   #84636

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub(TA)-YPet-NES
   

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR RPS27A K107/113R-3xFlag_IRES-GFP

    Plasmid

    #198392

    Purpose

    RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    ubiquitination site double mutant (K107/113R)

    Promoter

    EF1A

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO5d.SFFV.dTomato-miR30n

    Plasmid

    #90334

    Purpose

    miR30 based shMir knockdown

    Depositor

    Dirk Heckl

    Article

    Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

    Inserts

    dTomato
    na
    SFFV
    miR30n
    

    Use

    Lentiviral

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p-mCherry-C1-iCoChR++

    Plasmid

    #98173

    Purpose

    artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iCoChR++ gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    A59S, E83N, E90Q, E101S, Q117R, E123S, V242R, T24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1P POLE1_2

    Plasmid

    #160764

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_2
    

    Use

    Lentiviral

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL2 p15 (-756/+68) SBR2 Mut 3' box

    Plasmid

    #15711

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    p15 promoter (CDKN2B Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mut 3' box in SBR2. The 3' box contains…

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pSW683 - pAHP6>>

    Plasmid

    #115998

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. UbG76V-eGFP-V5His_15aa

    Plasmid

    #23969

    Depositor

    Nico Dantuma

    Article

    Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

    Insert

    Ub-G76V-eGFP-V5HIS_15aa (UBB Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    G76V mutation in Ubiquitin. Ub fused to N-termin…

    Available Since

    April 16, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-Tag2-Sox9 T236A/T240A

    Plasmid

    #111458

    Purpose

    Transient expression of Flag-tagged human Sox9 with T236A/T240A mutation

    Depositor

    Sharon Pine

    Article

    Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

    Insert

    Sox9 (SOX9 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T236A and T240A

    Promoter

    CMV

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5FRT/TO-PLAA-Strep-HA

    Plasmid

    #113484

    Purpose

    Expression of human PLAA with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    PLAA (PLAA Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-C-mNeon-Blast-TUBB4B

    Plasmid

    #207786

    Purpose

    Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. (p)odr-3::GFP::EGL-4

    Plasmid

    #100897

    Purpose

    GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

    Depositor

    Noelle L'Etoile

    Article

    Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

    Insert

    56-2741: odr-3 promoter
    

    Expression

    Worm

    Promoter

    odr-3

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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