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pEGFP-N1-PHD2

Plasmid

#21401

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

EGLN1 (EGLN1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pLKO human shRNA 1 DEPTOR

Plasmid

#21335

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

DEPTOR shRNA 1 (DEPTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
pBPGAL4.1Uw

Plasmid

#26226

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-sgRNA

Plasmid

#177783

Purpose

Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTrcAgBIS (CO)

Plasmid

#35153

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

Bisabolene Synthase

Use

Synthetic Biology

Expression

Bacterial

Promoter

pTrc

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-mCherry

Plasmid

#29585

Depositor

Anna Huttenlocher

Article

Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

Insert

mpx-mCherry

Use

Zebrafish expression

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Ndi-1/myc-His

Plasmid

#127503

Purpose

Expresses myc-tagged yeast Ndi-1 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

Ndi-1 (NDI1 Budding Yeast)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hPTGS2-2flag

Plasmid

#102498

Purpose

Mammalian expression of PTGS2

Depositor

Jun Yu

Article

Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

Insert

PTGS2 (PTGS2 Human)

Tags

Flag-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-sfCherry11-ß-actin

Plasmid

#70221

Purpose

Expresses sfCherry11-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11-actin (ACTB Human)

Tags

sfCherry11

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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peGFP(N1)-deltaCMV-rSyntaxin1a-meGFP

Plasmid

#34631

Depositor

Wolfhard Almers

Article

Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

Insert

rat Syntaxin1a (Stx1a Rat)

Tags

meGFP

Expression

Mammalian

Promoter

cmv

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pRST7-ER beta (long)

Plasmid

#11356

Depositor

Donald McDonnell

Article

Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

Insert

ER beta long (ESR2 Human)

Expression

Mammalian

Available Since

March 10, 2006

Availability

Academic Institutions and Nonprofits only
pJSK664

Plasmid

#205130

Purpose

Expression of GFP-2xPX (from SnxA) in mammalian cells

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

tandem 2xPX domain of SnxA

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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dimericTomato-2xrGBD

Plasmid

#176098

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Use

Lentiviral

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA

Plasmid

#105005

Purpose

Mammalian Expression of PDZD8

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-RSV-tdTomato-2A-GCaMP6f

Plasmid

#80317

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Inserts

tdTomato
GCaMP6f

Use

Lentiviral

Promoter

RSV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 1

Plasmid

#133770

Purpose

express 6Xhis- SUMO 1 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 1 (SUMO1 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAB46 (CK2beta)

Plasmid

#27085

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

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qs5

Plasmid

#24498

Depositor

Bruce Conklin

Article

Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

Insert

Gq alpha (Gnaq Mouse)

Tags

HA (see comments)

Expression

Mammalian

Mutation

Replacement of the five carboxyl-terminal amino a…

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
LentiGuide-puro-NLS-mCherry

Plasmid

#185474

Purpose

Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Type

Empty backbone

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pRPF215

Plasmid

#106377

Purpose

C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

Depositor

Robert Fagan, Neil Fairweather

Article

Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

Insert

Ptet-himar1-Ter(slpA)-transposon

Use

E. coli - c. difficile shuttle vector

Mutation

Codon optimised for C. difficile

Promoter

Ptet

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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