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PU6::klp-12_sgRNA

Plasmid

#46170

Purpose

klp-12 targeting sgRNA

Depositor

John Calarco

Publication

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

klp-12 targeting sgRNA (klp-12 Synthetic)

Use

CRISPR

Expression

Worm

Promoter

C. elegans U6 snRNA pol III promoter

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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RGS-SENP5

Plasmid

#18053

Depositor

Edward Yeh

Publication

Gong et al J Biol Chem. 2006 Jun 9. 281(23):15869-7

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SENP5 (SENP5 Human)

Tags

RGS

Expression

Mammalian

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pCMV5-ALK2 wt

Plasmid

#11741

Depositor

Jeff Wrana

Publication

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ALK2 (ACVR1 Human)

Expression

Mammalian

Available since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
FLAG.PKCzeta.K/W

Plasmid

#10800

Depositor

Alex Toker

Publication

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Kinase dead mutant: K281W (Lys 281 to Trp)

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
MLV Gag-YFP

Plasmid

#1813

Depositor

Walther Mothes

Publication

Sherer et al Traffic 2003 Nov;4(11):785-801.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gag (gag MuLV)

Tags

YFP

Expression

Mammalian

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcFLAG-FYN-WT

Plasmid

#74509

Purpose

Overexpression of FYN in mammalian cells

Depositor

Lars Rönnstrand

Publication

Chougule et al Oncotarget. 2016 Feb 2. doi: 10.18632/on

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FYN (FYN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-sfCherry11-ß-actin

Plasmid

#70221

Purpose

Expresses sfCherry11-ß-actin in mammalian cells

Depositor

Bo Huang

Publication

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sfCherry11-actin (ACTB Human)

Tags

sfCherry11

Expression

Mammalian

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-CDK19-WT-IRES-ZsGreen

Plasmid

#68858

Purpose

Expresses Human CDK19 Wild-Type

Depositor

Matthew Shair

Publication

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 19 (CDK19 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1α

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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MMK

Plasmid

#64121

Purpose

a modified episomal (EBNA1/OriP) vector expressing human Myc and KLF4 genes

Depositor

Linzhao Cheng

Publication

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MYC-2A-KLF4 (MYC, KLF4 Human)

Expression

Mammalian

Promoter

SFFV

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA6A-EGFR ECD (1-644)

Plasmid

#42666

Depositor

Mien-Chie Hung

Publication

Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

Insert

EGFR ECD (1-644) (EGFR Human)

Tags

6xHis and Myc

Expression

Mammalian

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-GlnRS-His

Plasmid

#124108

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutaminyl-tRNA ligase (glnS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pME-mCherry-FLAG-CD59-GPI

Plasmid

#50378

Purpose

Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cells

Depositor

Reika Watanabe

Publication

Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Expression

Mammalian

Promoter

SR alpha

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
AAV-oChIEF-citrine

Plasmid

#50974

Purpose

AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

Depositor

Roger Tsien

Publication

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

ChIEF gene is mammalian codon optimized

Promoter

human synapsin promoter

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP

Plasmid

#32628

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Sce

Use

Lentiviral

Available since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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223 pCS EGFP DEST

Plasmid

#13071

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MD-2

Plasmid

#20864

Depositor

Jie-Oh Lee

Publication

Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

Insert

Myeloid differentiation factor 2 (LY96 Human)

Tags

protein A tag

Expression

Insect

Mutation

contains amino acids 19–160

Available since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2W

Plasmid

#15809

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2W (UBE2W Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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RV-L3-HyTK-2L

Plasmid

#11684

Depositor

Geoff Wahl

Publication

Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAGD335

Plasmid

#219499

Purpose

pAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoter

Depositor

Seth Shipman

Publication

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K

Expression

Bacterial

Promoter

pBAD

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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