162,698 results

1. pET-pA-Hia5

   Plasmid

   #174372

   Purpose

   SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein A - hia5
   

   Use

   Tags

   6xHis and Protein A

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PH-PLCD1-GFP

   Plasmid

   #51407

   Purpose

   Is a biosensor for PI(4,5)P2

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   PH-PLCD1 (aa 1–170) (PLCD1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. c-myc-PT3EF1a

   Plasmid

   #92046

   Purpose

   Expresses c-Myc in mammalian cells

   Depositor

   Xin Chen

   Article

   Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

   Insert

   C-MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   July 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

   Plasmid

   #67977

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSIP403

   Plasmid

   #122028

   Purpose

   Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

   Depositor

   Geir Mathiesen, Lars Axelsson

   Article

   Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

   Insert

   gusA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sppA

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. gag/pol

   Plasmid

   #14887

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   gag / pol
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. YPet-N1

   Plasmid

   #54637

   Purpose

   Localization: N1 Cloning Vector, Excitation: 513, Emission: 527

   Depositor

   Michael Davidson, Patrick Daugherty

   Article

   Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

   Type

   Empty backbone

   Use

   Tags

   YPet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pMRX-No-HaloTag7-mGFP-LC3-mRFP

   Plasmid

   #184902

   Purpose

   Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Use

   Tags

   HaloTag7, monomeric EGFP, and monomeric RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LSL-Cas9-Rosa26TV

   Plasmid

   #61408

   Purpose

   Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   Cas9
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   3xFLAG and P2A

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CAG

   Available since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-Ikkb-FLAG WT

    Plasmid

    #23298

    Depositor

    Warner Greene

    Article

    Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

    Insert

    Ikkbeta (IKBKB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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