We narrowed to 29,779 results for: REP

1. LiRT

   Plasmid

   #214075

   Purpose

   Luminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Promoter

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LiRT_RTctrl

   Plasmid

   #214078

   Purpose

   Luminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Promoter

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LiRTi

   Plasmid

   #214076

   Purpose

   Luminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Promoter

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LiRTX_ctrl

   Plasmid

   #214077

   Purpose

   Luminescent reporter system, NMD negative control. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Remnant of linker sequence present at the end of …

   Promoter

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYIC

   Plasmid

   #18673

   Depositor

   Han Htun

   Article

   Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

   Type

   Empty backbone

   Use

   Tags

   3Myc-EYFP-HA-His6 and ECFP-HA-His6

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-ERK-SPARK

   Plasmid

   #106921

   Purpose

   Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   ERK-SPARK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSCW01

   Plasmid

   #72300

   Purpose

   Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Type

   Empty backbone

   Use

   Derivative of puc19

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPomyces-SaCas9

   Plasmid

   #129553

   Purpose

   Contains codon-optimized SaCas9 and gRNA for streptomyces

   Depositor

   Mingzi M. Zhang, Fong Tian Wong

   Article

   Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

   Insert

   SaCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ARTi

   Plasmid

   #214064

   Purpose

   Fluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   TurboRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Linker sequence present between RFP and GFP

   Promoter

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ART

    Plasmid

    #214063

    Purpose

    Fluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between RFP and GFP

    Promoter

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPomyces-FnCpf1

    Plasmid

    #129554

    Purpose

    Contains codon-optimized FnCpf1 and gRNA for streptomyces

    Depositor

    Mingzi M. Zhang, Fong Tian Wong

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    FnCpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGilesSHdI_psmyc_His6_triple red

    Plasmid

    #170047

    Purpose

    Mono-Responsive Reporter Gene Constructs for triple red

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet
    COmScarlet
    mRuby3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNOC-stacked-GFP-aequorin

    Plasmid

    #101011

    Purpose

    Construct for expression of a calcium sensitive luciferase reporter, GFP-aequorin, and hygromycin resistance gene by a bidirectional promoter

    Depositor

    Eva Farre

    Article

    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Tags

    Expression

    Mutation

    Promoter

    Ribi

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FDP-P10B2-A75-RZ-URA3

    Plasmid

    #130874

    Purpose

    Integration cassette for OrthoRep containing mKate driven by promoter 10B2 with a hardcoded polyA tail

    Depositor

    Chang Liu

    Article

    Zhong et al ACS Synth Biol. 2018 Dec 21;7(12):2930-2

    Insert

    mKate
    

    Use

    Expression of mkate, leu2(q180*), and ura3 on ort…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-tetO-Oct4

    Plasmid

    #19766

    Depositor

    Konrad Hochedlinger

    Article

    Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ART_RTctrl

    Plasmid

    #214066

    Purpose

    Fluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between RFP and GFP

    Promoter

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6-Universal

    Plasmid

    #52694

    Purpose

    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

    Depositor

    Sebastian Lourido

    Inserts

    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    

    Use

    CRISPR; Toxoplasma gondii

    Tags

    3X FLAG and Nuclear localization signal

    Expression

    Mutation

    Promoter

    TgTUB1 and Toxoplasma U6

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKIR1.0

    Plasmid

    #86409

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    AtRPS5A

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNOC-ARS-stacked-NeoR-mNeonGreen

    Plasmid

    #101249

    Purpose

    Construct for fusion expression of mNeonGreen reporter and G418 resistance gene by a bidirectional promoter

    Depositor

    Eva Farre

    Article

    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Tags

    Expression

    Mutation

    Promoter

    Ribi

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs-Hu-L-Myc

    Plasmid

    #26022

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    L-Myc (MYCL Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FUW-KO

    Plasmid

    #20331

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Klf4-F2A-Oct4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG-Pol-II 1-22

    Plasmid

    #35178

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal domain repeats 23-52

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

23. FLAG-Pol-II 31-52

    Plasmid

    #35179

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal domain repeats 1-30

    Promoter

    CMV

    Available Since

    Oct. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

24. WT-Pan

    Plasmid

    #99638

    Purpose

    EGFP-mCherry control reporter for methionine misincorporation

    Depositor

    Tao Pan

    Article

    Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

    Insert

    mCherry
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCTCON2-GFP

    Plasmid

    #158130

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AiRTi

    Plasmid

    #214068

    Purpose

    Fluorescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between RFP and GFP

    Promoter

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AiRT

    Plasmid

    #214067

    Purpose

    Fluorescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between RFP and GFP

    Promoter

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCRISPomyces-AsCas12j-2

    Plasmid

    #191655

    Purpose

    All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

    Depositor

    Fong Tian Wong

    Article

    Tan et al bioRxiv 2021.10.28.465406

    Insert

    AsCas12j-2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CSII-prEF1a-E2-Crimson-NLS

    Plasmid

    #125263

    Purpose

    Fluorescent reporter for cell size

    Depositor

    Jan Skotheim

    Article

    Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

    Insert

    E2-Crimson-NLS
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL2_163

    Plasmid

    #199095

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Cultivarium Tools, Nili Ostrov, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pAMβ1 ORI plus NGS barcode 3, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge