We narrowed to 25,556 results for: lars
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Plasmid#27710DepositorAvailable SinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only
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pLO99
Plasmid#90219PurposeComplements mutations in Aspergillus nidulans gene lysB (AN5206) (mutants require lysine)DepositorInsertATET_03691
UseAspergillusPromoterpLacAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CaM-V5-sTb(N)
Plasmid#219779PurposesTb(N) half of CaST for HEK cell expressionDepositorInsertCaM-V5-sTb(N)
UseAAVTagsV5ExpressionMammalianPromoterCMVAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTmpGm
Plasmid#176229PurposeA template plasmid for amplification of the cI-hok-aacC4 cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, aacC4
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
zCD2
Plasmid#122031PurposeExpresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.DepositorInsertZebrafish HDAC6 Catalytic Domain 2
TagsHis6-MBP tag with cleavable TEV siteExpressionBacterialPromoterT7Available SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+18)
Plasmid#199162Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+18)
TagsMGHHHHHHGGAMutationE16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)
Plasmid#48295DepositorTypeEmpty backboneTagsHis6ExpressionInsectAvailable SinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
POM43-mTFP1
Plasmid#69853PurposeN-terminal tagging with mTFP1 using LEU2 as a selection marker in S. cerevisiae.DepositorInsertmTFP1 minus start and stop codon
ExpressionYeastAvailable SinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+12)
Plasmid#199161Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+12)
TagsMGHHHHHHGGAMutationE16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
TOPO GFP
Plasmid#68488PurposeTOPO construct expressing GFP for generation of GMAP-compatible geneDepositorInsertGreen Fluorescent Protein
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJUMP27ts-1A(sfGFP)
Plasmid#126975PurposeLevel 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationRepA A56V thermosensitive mutationAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-p23
Plasmid#108225PurposeMammalian expression vector for EYFP fusion to human p23DepositorAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG 3.1
Plasmid#193802PurposeProtein production of ChemoG3.1 in bacteriaDepositorInsertChemoG 3.1
Tags10xHisExpressionBacterialMutationEGFP: A206K, T225RPromoterT7Available SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2-EM7neoPheS
Plasmid#112923PurposepiggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)DepositorInsertneoPheS
UsePiggybac transposon gateway destination vectorExpressionMammalianAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA HA FAK Y407F
Plasmid#50504DepositorInsertprotein tyrosine kinase, mutated
TagsHAExpressionMammalianMutationY407FPromoterCMVAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)
Plasmid#48297DepositorTypeEmpty backboneTagsStrepII and msfGFPExpressionInsectAvailable SinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pY2-MET-IV
Plasmid#218117PurposeMET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-LYS-IV
Plasmid#218118PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PTBP1-Halo
Plasmid#175302PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMV261-Cas12a
Plasmid#158707Purposeexpresses codon-optimized FnCpf1DepositorInsertFnCpf1
ExpressionBacterialPromoterTetOAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Myc-His(-)-MSulf-1
Plasmid#13007DepositorInsertmouse sulfatase 1 (Sulf1 Mouse)
ExpressionMammalianAvailable SinceOct. 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest53-SHP-1 WT
Plasmid#21791DepositorAvailable SinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJBL701
Plasmid#140371PurposeExpresses TetR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-TetR-T
Tags6XHisExpressionBacterialAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ACTG1_Actin
Plasmid#109989PurposeProtein expression and purification of ACTG1_ActinDepositorInsertACTG1_Actin
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBLego
Plasmid#219967PurposeThe pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.DepositorTypeEmpty backboneTagsSLEHHHHHH and pelB signal sequenceExpressionBacterialPromoterpBADAvailable SinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
MDC1 KO sgRNA
Plasmid#207103PurposepX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.DepositorInsertGGTGTAACGTGGAGCCAGTA
ExpressionMammalianPromoterCMV and U6Available SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-4493NES(Booster-PKA)
Plasmid#184709PurposePKA biosensorDepositorInsertBooster-PKA
ExpressionMammalianAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)
Plasmid#66708Purposeexpresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removedDepositorInsertS.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64MutationD10A and H840APromoterhUbCAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCL28-decoder
Plasmid#159450PurposeInsert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.DepositorInsertPlexAtet-LexA-ER-B112-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL66-decoder
Plasmid#159453PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only