We narrowed to 5,907 results for: arl

1. pTet-O-LHX8-T2A-PuroR

   Plasmid

   #162350

   Purpose

   Lentiviral expression of LHX8 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   LHX8 (LHX8 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Promoter

   Tet-ON

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

   Plasmid

   #35499

   Purpose

   AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Has Service

   AAV9

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP

   Plasmid

   #35497

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Has Service

   AAV9

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

   Plasmid

   #35504

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   hChR2(C128S/D156A)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C128S and D156A

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. GLuc-PM-Venus

   Plasmid

   #164785

   Purpose

   Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Gaussia princeps, Human)
   

   Tags

   GLuc and mVenus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYTSK01K_0G7

   Plasmid

   #177296

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

   Depositor

   Karl-Erich Jaeger

   Article

   Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

   Insert

   PaacC1-aacC1
   

   Use

   Synthetic Biology; Yeast expression, tn7 genomic …

   Expression

   Bacterial

   Available Since

   Aug. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUFlip-floxed2A-KalTA4-; HE1A:BFP -0

   Plasmid

   #180010

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; HE1A: BFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. G-GECO1-Orai1

   Plasmid

   #73561

   Purpose

   Green fluorescent reporter of Orai1-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   NM_032790.3 (ORAI1 Synthetic, Human)
   

   Tags

   G-GECO1

   Expression

   Mammalian

   Promoter

   CMV Immediate Early

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

   Plasmid

   #35509

   Purpose

   AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Has Service

   AAV5 and AAV9

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C1 EDD

    Plasmid

    #37190

    Depositor

    Darren Saunders, Charles Watts

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD (UBR5 Human)
    

    Tags

    6x His Tag and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry

    Plasmid

    #35502

    Purpose

    AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2(C128S/D156A)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

    Plasmid

    #180011

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn Con/Foff EYFP

    Plasmid

    #55651

    Purpose

    Cre-on/Flp-off EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    eYFP with introns
    

    Use

    AAV; Cre on/flp off eyfp

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-CAD

    Plasmid

    #73566

    Purpose

    Soluble red fluorescent activator of Orai1 channels

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

    Insert

    mCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)
    

    Tags

    6x His and mCherry

    Expression

    Mammalian

    Promoter

    CMV Immediate Early

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSG5 EDD

    Plasmid

    #37191

    Depositor

    Darren Saunders, Charles Watts

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD (UBR5 Human)
    

    Tags

    6x His Tag and FLAG

    Expression

    Mammalian

    Mutation

    K503R (please see depositor comments below)

    Promoter

    SV40

    Available Since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTet-O-E2F7-T2A-PuroR

    Plasmid

    #162348

    Purpose

    Lentiviral expression of E2F7 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Promoter

    Tet-ON

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EF1a-FDIO-ArchT-GFP

    Plasmid

    #124640

    Purpose

    AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    ArchT
    

    Use

    AAV; Flp/frt

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJW2072

    Plasmid

    #154334

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-nEF-NpHR3.3-EYFP

    Plasmid

    #137151

    Purpose

    Viral expression of NpHR3.3-EYFP

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    NpHR3.3-EYFP
    

    Use

    AAV

    Mutation

    W179F

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTet-O-KLF7-T2A-PuroR

    Plasmid

    #162346

    Purpose

    Lentiviral expression of KLF7 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    KLF7 (KLF7 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Promoter

    Tet-ON

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge