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pNL-CEF-IgA1

Plasmid

#187009

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

Use

Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-p27Kip1 3'UTR

Plasmid

#29476

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

p27 Kip1 3'UTR (CDKN1B Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pRS-mTagRFP-TUBA1B

Plasmid

#62850

Purpose

Donor vector for gene editing at human TUBA1B locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

mTagRFP-TUBA1B (TUBA1B Synthetic, Human)

Use

Yeast-e.coli shuttle vector

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pmKikGR Actin

Plasmid

#105315

Purpose

Photoconvertable cytoskeletal protein actin to study kinetics or dynamics

Depositor

Kenneth Yamada

Article

Doyle et al J Cell Sci. 2012 May 1;125(Pt 9):2244-56

Insert

actin beta (ACTB Human)

Tags

KikGR

Expression

Mammalian

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ Flag-RAGB99L

Plasmid

#128101

Purpose

Expresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

Ras related GTP binding B (RRAGB Human)

Tags

Flag

Expression

Mammalian

Mutation

Q99L (CAA to CTA)

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Cav2.2 D122A HA pCAGGS

Plasmid

#206097

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

D122A

Promoter

CMV/B-actin

Available Since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA D122A pcDNA3

Plasmid

#206108

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

D122A

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-IgM

Plasmid

#187013

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM

Use

Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE

Plasmid

#87919

Purpose

HIV-1 SIN lentiviral transfer vector

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgHTT1, shGFP2, sgCas9, cherry

Use

Lentiviral

Promoter

H1, U6, 7SK, PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_01

Plasmid

#158102

Purpose

5'UTR stem-loop luciferase reporter: 0kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop 0kcal/mol

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-E2F3 3'UTR

Plasmid

#29470

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

E2F3 3'UTR (E2F3 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
psiCHECK2-CHEK1 3'UTR

Plasmid

#29478

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

CHEK1 3'UTR (CHEK1 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
Nsp14D-mCherry

Plasmid

#165139

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp14D (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp4 deltaC-EGFP

Plasmid

#165126

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp4 deltaC (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgQKI-2201

Plasmid

#115449

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgQKI-2201 (QKI Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgQKI-2204

Plasmid

#115450

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgQKI-2204 (QKI Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)

Plasmid

#174336

Purpose

The ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human Orai1 (ORAI1 Human)

Use

Retroviral

Tags

pHluorin inserted in the extracellular loop 2 of …

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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human TLNRD1-F250D pET151

Plasmid

#159385

Purpose

Expresses human TLNRD1 F250D mutant in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Changed Phenylalanine 250 to Aspartic Acid (F250D)

Promoter

T7

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_02

Plasmid

#158103

Purpose

5'UTR stem-loop luciferase reporter: -30kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop -30kcal/mol

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp2-a del.SHD

Plasmid

#40050

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a del.SHD (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of SHD domain (1-56)

Promoter

CMV promoter

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only