We narrowed to 9,326 results for: sacs

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pFA6a-3HA-His3MX6

Plasmid

#41600

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211463

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-dead

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D211A, E225A, K227A

Promoter

TRE

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCAM-kan-R4R3

Plasmid

#71275

Purpose

Binary destination vector for plant transformation. Kanamycin resistance.

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Type

Empty backbone

Use

Multisite gateway

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pBF3060

Plasmid

#26853

Purpose

Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Insert

CreA recombinase

Use

Cre/Lox

Expression

Yeast

Mutation

N/A

Promoter

GAL1

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pJCSUP35(1-253)

Plasmid

#1089

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

amino acids 1 - 253 of SUP35

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pBD-pZ-FLAG

Plasmid

#183148

Purpose

Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM)

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

GAL4 BD

Use

Yeast two-hybrid

Tags

FLAG

Expression

Yeast

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pZH82 pET28a-Rai1

Plasmid

#231651

Purpose

Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

rai1 (RAI1 Budding Yeast)

Expression

Bacterial

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pZH23 Dual G-less containing ySSA1 polyA

Plasmid

#231647

Purpose

yeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassette

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

SSA1 polyA termination region

Use

Synthetic Biology

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pZH20 Dual G-less containing yCYC1 polyA

Plasmid

#231646

Purpose

yeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassette

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

CYC1 polyA termination region

Use

Synthetic Biology

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pEx-deletion-erm

Plasmid

#172213

Purpose

For allelic exchange in erythromycin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Allelic exchange in prevotella copri

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP(S65T)-kanMX6

Plasmid

#39292

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only

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p2R3z-AtU3b-tRNA-ccdB-gRNA

Plasmid

#118390

Purpose

Entry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

AtU3b promoter

Use

CRISPR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pR26 GFP Dest

Plasmid

#74283

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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IRE1-2EGFP-ter-kanMX4

Plasmid

#184766

Purpose

Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

IRE1 (IRE1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pACT-Lex

Plasmid

#183149

Purpose

Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

GAL4 AD

Use

Yeat two-hybrid

Tags

HA tag

Expression

Yeast

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-EGFP-KASH

Plasmid

#154374

Purpose

Vector for Flp-dependent expression of EGFP tagged KASH protein domain

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

EGFP-KASH

Use

AAV

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pYSD063

Plasmid

#212916

Purpose

Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-CMV-TFAM-BioID2-IRES-Puro

Plasmid

#211465

Purpose

Lentivirus backbone expressing human TFAM fused with BioID2

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

TFAM-BioID2

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLPCX-NICD

Plasmid

#44471

Depositor

Ernesto Canalis

Article

Zanotti et al J Biol Chem. 2011 Feb 11;286(6):4576-88.

Insert

Notch intracellular domain (Notch1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Contains amino acid 1753 to C-Term

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only