We narrowed to 15,732 results for: LEA

1. GFP-p38beta

   Plasmid

   #86833

   Purpose

   mammalian expression of GFP-p38beta

   Depositor

   Rony Seger

   Article

   Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

   Insert

   p38beta (MAPK11 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDFNKI-strepII-3C-LIC-strep

   Plasmid

   #108708

   Purpose

   Expression of a StrepII-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Tags

   StrepII-3C protease cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRARE-MBP-DEST

   Plasmid

   #84650

   Purpose

   Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

   Depositor

   Neil Olszewski

   Article

   Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

   Type

   Empty backbone

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

   Plasmid

   #182415

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-DARPinOFF7
   

   Tags

   Vesicle Nucleating peptide (VNp) & mNeongreen

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP - FLAG-APEX2- hs Lamin B1

   Plasmid

   #139442

   Purpose

   APEX2-Lamin B1 expression plasmid

   Depositor

   Yixian Zheng

   Article

   Tran et al bioRxiv 2020.02.05.935635

   Insert

   LMNB1 (LMNB1 Human)
   

   Tags

   3xGGGGS linker (between APEX2 and Lamin B1), APEX…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-Flag-Tpr

   Plasmid

   #60882

   Purpose

   Expresses Flag-Tagged nucleoporin Tpr in Mammalian Cells

   Depositor

   Vinay Nandicoori

   Article

   Vomastek et al Mol Cell Biol. 2008 Nov;28(22):6954-66.

   Insert

   Tpr (TPR Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. TetO-FUW-PBRM1-pgk-puro

   Plasmid

   #85746

   Purpose

   tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

   Depositor

   Emily Dykhuizen

   Article

   Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

   Insert

   PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   none

   Promoter

   CMV

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-JNK2

   Plasmid

   #86831

   Purpose

   mammalian expression of GFP-JNK2

   Depositor

   Rony Seger

   Article

   Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

   Insert

   JNK2 (MAPK9 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. CyPET-Arf6

   Plasmid

   #18840

   Depositor

   Martin Schwartz

   Article

   Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

   Insert

   CyPET-Arf6 (ARF6 Human)
   

   Expression

   Mammalian

   Mutation

   The sequence for the fluorescent protein CyPET wa…

   Available Since

   Aug. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-HA-CUL1 Y42A/M43A

    Plasmid

    #19940

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 Y42A/M43A (CUL1 Human)
    

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    CUL1 with a SKP1 binding mutant

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

11. BPK2101

    Plasmid

    #65770

    Purpose

    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS-3xFlag

    Expression

    Bacterial

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6598-HisSUMO1GG

    Plasmid

    #13376

    Depositor

    Anne Dejean

    Article

    Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    T7 in vitro translation

    Tags

    His

    Expression

    Mammalian

    Mutation

    mature form ending with GG

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSP680

    Plasmid

    #65772

    Purpose

    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid

    #222099

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Vitiosangium-bGSDM-thrombin ORF
    

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETSUMO2_Nostoc-bGSDM-WELQ

    Plasmid

    #222100

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Nostoc-bGSDM-WELQ ORF
    

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLUT7 HA-GLI1

    Plasmid

    #62970

    Purpose

    Expresses HA-tagged Gli1

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP712

    Plasmid

    #65768

    Purpose

    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.RMacL3-01.EGFP.BCA5.WPRE

    Plasmid

    #225685

    Purpose

    L3 pyramidal neuron Enhancer to drive transgene expression

    Depositor

    William Stauffer

    Article

    He et al Neuron. 2025 May 21;113(10):1548-1561.e8

    Insert

    RMacL3-01.EGFP.BCA5
    

    Expression

    Mammalian

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-GRAB_OTmut

    Plasmid

    #185388

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Has Service

    AAV1 and AAV9

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330 Human 3' HP1a gRNA

    Plasmid

    #127907

    Purpose

    WT Cas9 Vector targeting the 3' end of the human HP1a gene

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    gRNA for Human 3' HP1a
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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