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pSR49

Plasmid

#69259

Purpose

phlh-8::tagBFP2

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

TagBFP2

Use

Cre/Lox

Expression

Worm

Mutation

codon-optimzed index 1.0, TagBFP2 contains 2 sing…

Promoter

hlh-8

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Ef1a_NANOG_Ires_Puro

Plasmid

#18920

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

NANOG (NANOG Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB2-SA(1)-T2A-Gal4

Plasmid

#62900

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-Gal4

Plasmid

#62901

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-Venus

Plasmid

#87613

Purpose

Actin-targeted Venus (YFP).

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-Venus

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ALPPL2 synNotch

Plasmid

#188376

Purpose

Lentiviral vector - constitutive expression of an anti-ALPPL2 synNotch with a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiALPPL2_synNotch_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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V1-MESA-45F-M-tTA

Plasmid

#84500

Purpose

MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and tTA

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V1-MESA-45F-M-tTA

Use

AAV

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2

Plasmid

#79902

Purpose

Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

3xFLAG_Twin_Strep_EZH2

Use

CRISPR and TALEN

Tags

3xFLAG_Twin_Strep

Expression

Mammalian

Promoter

PGK1

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_ADAM17 site

Plasmid

#188349

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a ADAM17site, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_ADAM17site_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 1-1290

Plasmid

#78579

Purpose

Expression flag-USP19 1-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-1290 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutate 1291 residue to stop condon

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
USP19 sgRNA2

Plasmid

#78586

Purpose

delete USP19 gene in human cell

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 sgRNA2 (USP19 Human)

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pDendra2-H2a

Plasmid

#157816

Purpose

Fluorescent reporter for histone H2A tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H2A (H2ac13 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR4_PRKRA

Plasmid

#106110

Purpose

Entry vector for PRKRA

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Gateway entry vector

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 uGFP

Plasmid

#14557

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pENTR2B-dCas9-KRAB

Plasmid

#115547

Purpose

Entry cassette for dCas9-KRAB

Depositor

Rene Maehr

Article

Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

Insert

dCas9-KRAB

Tags

HA

Expression

Bacterial and Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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RFP-(SUMO FAAA)3

Plasmid

#130703

Purpose

Mammalian expression plasmid for negative control of mCherry-(SUMO)3.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

3 copies of human SUMO1 separated by (GGS)4

Expression

Mammalian

Mutation

FKVK sequence in each SUMO1 module changed to FAAA

Promoter

CMV

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-Otx2shRNA1

Plasmid

#73979

Purpose

Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Otx2 gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta1

Plasmid

#106114

Purpose

Lentiviral expression of PRKRA delta1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK (FLAG) and myc

Mutation

AA 100-313

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSR40

Plasmid

#69257

Purpose

plin-31::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

lin-31

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-Inpp5e

Plasmid

#96808

Purpose

cilia-membrane-targeted catalytic domain of inositol phosphae 5-phosphatase, CAAX-null

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-Inpp5e

Tags

YFP

Expression

Mammalian

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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