171,784 results
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Plasmid#128611Purposea spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.DepositorInsertsmHA MF tag
ExpressionMammalianAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-Best1-sCX3CL1
Plasmid#164422PurposeAAV plasmid expressing soluble CX3CL1 (fractalkine) in retinal pigment epitheliumDepositorInsertCx3cl1 (Cx3cl1 Mouse)
UseAAVExpressionMammalianMutationdeleted terminal 59 amino acidsPromoterBest1Available SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a-ABCD3-RFP-Puro
Plasmid#170168PurposeMammalian Expression of Ef1a-ABCD3-RFP-PuroDepositorInsertABCD3-RFP-Puro
ExpressionMammalianMutationn/aAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
SF-SON IRES Tomato
Plasmid#214403PurposeExpresses human SOX10, OLIG2, NKX6.2 and tdTomatoDepositorUseLentiviralExpressionMammalianPromoterSFFVAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1
Plasmid#12209DepositorAvailable SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUD-MSP1D1
Plasmid#161608PurposeBacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
Tags7-His tagExpressionBacterialMutationmutant of MSP1D1; all tryptophans and tyrosines w…PromoterT7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-hygro-EGFP-Stx17
Plasmid#174652PurposeVector for overexpression of EGFP-tagged Stx17 with IRES for hygromycin selection.DepositorAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
G9A[SET]-dCas9
Plasmid#100089PurposeCatalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertG9A (EHMT2 Human)
UseCRISPRTags3XFLag-NLS-G9A[SET]-dCas9-NLSExpressionMammalianMutationG9A[SET] includes aa 829-1209PromoterCMVAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-UPRT-T2A-RFP-IRES-CD
Plasmid#126677PurposeDoxycycline inducible expression of UPRT and CD along with reporter RFPDepositorInsertUPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
Promotertight TREAvailable SinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
iC13 PB-TO-oNgn2-BFP-puro
Plasmid#204733PurposeImproved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.InsertOptimized neurogenin-2
ExpressionMammalianAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
hKCNQ5(WT).ires.GFP-pcDNA3
Plasmid#204360PurposeHeterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)DepositorAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-FLAG-Jhdm2a
Plasmid#38136DepositorInsertJhdm2a (Kdm3a Mouse)
TagsFLAGExpressionMammalianMutationprotein starts at aa115PromoterCMVAvailable SinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFGAMS-mCherry
Plasmid#166024PurposeExpresses human PFAS-mCherry fusion in mammalian cellsDepositorAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pQLinkN
Plasmid#13670DepositorInsertmulti cloning site
UseCo-expressionExpressionBacterialAvailable SinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.1.t1
Plasmid#73547PurposetRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.1.t1
pAzFRS.1.t1
ExpressionBacterialAvailable SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
WRN scFv [N428/12]
Plasmid#206784PurposeMammalian Expression of WRN scFV. Derived from hybridoma N428/12 scFv.DepositorAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0500
Plasmid#143709PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
5KKP (PUS7)
Plasmid#101878PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-link-ymYPET-URA3
Plasmid#168056PurposeReplaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymYPET
TagsymYPETExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB117 - pL2_pSB90_fLUC-I_V2
Plasmid#123192Purposebinary plant vector for transient TYLCV V2 and fLUC (with intron) expressionDepositorInsert2x35S::V2::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseExpressionPlantAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
a1Ha-pcDNA3
Plasmid#45809DepositorAvailable SinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYH80
Plasmid#217387PurposePtrc99A-mCherry-L78-PopTag for bacterial expressionDepositorInsertPopTag
TagsmCherry-Linker78ExpressionBacterialAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNL-EGFP/TREPittdU3
Plasmid#18659DepositorInsertEGFP
UseLentiviralAvailable SinceJuly 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-ALFA-His
Plasmid#171569PurposeBacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)DepositorInsertNbVHH05-ALFA-His (UBE2J1 Human)
TagsALFA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCnCas12f1HS_sgRNA_MS13_empty
Plasmid#204999PurposeCnCas12f1 with MS13 sgRNA site for Human cell genome editingDepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCas9_GFP
Plasmid#44719PurposeCo-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cellsDepositorInsertCas9-2A-GFP
UseCRISPRTags2A-GFPExpressionMammalianPromoterCAGAvailable SinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pCMV-AcGFP
Plasmid#131008PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianPromoterpCMV and pTRE3G-BiAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pg-HIF-1alpha-EGFP
Plasmid#87204PurposeC-terminally EGFP-tagged human HIF-1aDepositorAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only