163,606 results

1. TRUPATH Triple Ga13

   Plasmid

   #196062

   Purpose

   Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha13-RLuc8 (GNA13 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD649-HAsp-AGER-COMP5AP-AviTag-9xHis

   Plasmid

   #157325

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   AGER (AGER Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5E-elavl3

   Plasmid

   #82400

   Purpose

   Gateway-compatible zebrafish elavl3 promoter

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   elavl3 promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV CAMKII PSAM4 GlyR IRES EGFP

   Plasmid

   #119744

   Purpose

   Chemogenetic inhibitor expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Has Service

   AAV5

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   CamKII

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-SpCas9-GFP-U6-gRNA

   Plasmid

   #79144

   Purpose

   All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

   Depositor

   Jizhong Zou

   Article

   Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMpGWB104

   Plasmid

   #68558

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   GUS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAID1.2-EF1a-CGFP-mAID

   Plasmid

   #140608

   Purpose

   Expression of OsTIR1 and mAID-GFP protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PER2:Luc Plasmid

   Plasmid

   #206875

   Purpose

   Per2 promoter luciferase reporter

   Depositor

   Michelle Farkas

   Article

   Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

   Insert

   Period 2 (Per2 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SynTagMA_post

   Plasmid

   #119736

   Purpose

   Synaptic Tag for Mapping Activity

   Depositor

   Thomas Oertner

   Article

   Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

   Insert

   SynTagMA
   

   Use

   AAV

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn-1

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2lox-cAMPr

    Plasmid

    #99143

    Purpose

    Expresses cAMPr in mammalian cells

    Depositor

    Justin Blau

    Article

    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

    Insert

    cAMPr
    

    Use

    Cre/Lox

    Tags

    cpGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMTU-DO-LEU

    Plasmid

    #160006

    Purpose

    Low-copy expression vector for K. marxianus, leucine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_BiSSTe4_Gq_dTomato

    Plasmid

    #213946

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCiES

    Plasmid

    #60010

    Purpose

    Foamy virus helper plasmid expressing Env, driven by a CMV promoter. Ampicillin resistant.

    Depositor

    David Russell

    Article

    Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

    Insert

    Env (foamy virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-AsCas12f1

    Plasmid

    #171614

    Purpose

    AsCas12f1-based Human cell genome editing

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1 and sgRNA_v1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBABEpuro HA Brca1

    Plasmid

    #14999

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

    Insert

    Brca1 (BRCA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCiGSΔΨ

    Plasmid

    #108312

    Purpose

    Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.

    Depositor

    David Russell

    Article

    Josephson et al Hum Gene Ther. 2004 Jan;15(1):87-92.

    Insert

    gag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    pCiGSΔΨ is a newer version of the pCiGS Gag expre…

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Flpo

    Plasmid

    #60662

    Purpose

    Expresses pCAG-Flpo; codon-optimized for mammalian expression

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Flpo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDY1623 NOLC1 sgRNA 2

    Plasmid

    #234834

    Purpose

    sgRNA 2 for NOLC1 STITCHR insertion

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    NOLC1 sgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3xFLAG-CMV10-mApg16L

    Plasmid

    #24302

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

    Insert

    autophagy related genes 16L (Atg16l1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    V583M

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-hSyn-ASAP4e-Kv-WPRE

    Plasmid

    #201031

    Purpose

    Expresses somatically enriched ASAP4e in neurons, can be used to package AAV

    Depositor

    Michael Lin

    Article

    Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

    Insert

    ASAP4e-Kv
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBSV2_PresT-mCherryBb

    Plasmid

    #118238

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    resT promoter driven mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pmRFP-LC3

    Plasmid

    #21075

    Purpose

    Mammalian expression of rat LC3 fused to mRFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    First ATG is not included.

    Promoter

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDONR223-SRC

    Plasmid

    #23934

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    SRC (SRC Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pOmniBac zz TEV YBBR TRF2

    Plasmid

    #185443

    Purpose

    Expresses human TRF2 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Insert

    TRF2 (TERF2 Human)
    

    Use

    Tags

    YBBR and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCas9-CR4

    Plasmid

    #62655

    Purpose

    Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

    Depositor

    Kristala Prather

    Article

    Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

    Insert

    Cas9 nuclease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

    Plasmid

    #129631

    Purpose

    Visualization of Akt and ERK activity

    Depositor

    Dorus Gadella

    Article

    Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

    Insert

    H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLys-M1 backbone

    Plasmid

    #109386

    Purpose

    Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmR

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pC0090

    Plasmid

    #181954

    Purpose

    Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    7xTOPFlash
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CROPseq-tRFP

    Plasmid

    #230938

    Purpose

    Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

    Depositor

    Hyejung Won

    Article

    Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagRFP

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. cytoplasmic cpVenus control

    Plasmid

    #186788

    Purpose

    Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    cytoplasmic cpVenus control
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge