We narrowed to 9,942 results for: Uty

1. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP

   Plasmid

   #207862

   Purpose

   AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP
   

   Use

   AAV

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. A24M04 mIgM heavy chain-pFEEKW

   Plasmid

   #234809

   Purpose

   Express membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

   Depositor

   Susan Pierce

   Article

   Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

   Insert

   A24M04 membrane IgM heavy chain (IGHM Human)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Promoter

   EEK promoter

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. kiCAP-AAV-MyoAAV3A

   Plasmid

   #224442

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3A, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDYVGL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. kiCAP-AAV-MyoAAV4E

   Plasmid

   #224452

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDFNNT insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM-GFP-URA3-GFP

   Plasmid

   #72606

   Purpose

   Template for creating a C-terminal GFP tag AND an internal GFP tag from a single transfection of yeast

   Depositor

   Bruce Granger

   Article

   Granger et al PLoS One. 2018 Jan 12;13(1):e0191194. do

   Inserts

   GFP (full)
   URA3
   GFP (partial)
   Optional Linker
   

   Mutation

   Includes full 819 bp coding sequence of URA3, 435…

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. A24M04 Ig kappa light chain-pFEEKW

   Plasmid

   #234810

   Purpose

   Express Ig kappa light chain containing the VL region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

   Depositor

   Susan Pierce

   Article

   Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

   Insert

   A24M04 Igkappa light chain (IGKC, IGK Human)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Promoter

   EEK promoter

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. plx304-PHB-V5

   Plasmid

   #122230

   Purpose

   Expresses human PHB attached to a V5 tag

   Depositor

   Alex Kentsis

   Article

   Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

   Insert

   Prohibitin (PHB1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHRIG-Akt3

   Plasmid

   #53596

   Purpose

   lentiviral expression of constitutively active human Akt3 and EGFP

   Depositor

   Heng Zhao

   Article

   Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

   Insert

   cAkt3 (AKT3 Human)
   

   Use

   Lentiviral

   Tags

   HA and IRES-EGFP

   Expression

   Mammalian

   Mutation

   constitutively active (myristoylated version)

   Promoter

   CMV

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pAAVtri-CMV-eGFP

    Plasmid

    #230932

    Purpose

    Used for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes.

    Depositor

    Qizhao Wang

    Article

    Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

    Insert

    eGFP
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Archon1-KGC-EGFP-ER2-WPRE

    Plasmid

    #108423

    Purpose

    Archon1 fluorescent voltage reporter in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

    Plasmid

    #223159

    Purpose

    Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CA-RIT-NFAT1 (IRES-GFP)

    Plasmid

    #85181

    Purpose

    Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selection

    Depositor

    Anjana Rao

    Article

    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

    Insert

    CA-RIT-NFAT1 (Nfatc2 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Amino acids 1-3 removed; CA: PASSGSSASF mutated t…

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pHLS-EF1a-FRB-SpCas9-A

    Plasmid

    #138477

    Purpose

    Expresses FRB fused SpCas9 for NanoMEDIC packaging.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    SpCas9, human codon optimized
    

    Use

    CRISPR

    Tags

    3x HA tag and FRB

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-Tet-MKK6(DD)-Puro

    Plasmid

    #86094

    Purpose

    Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

    Depositor

    Cindy Miranti

    Article

    Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Serine 207 and Threonine 211 both changed to Aspa…

    Promoter

    CMV/TetO

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1

    Plasmid

    #212522

    Purpose

    Encodes SARS-CoV-2 variant XBB.1 Spike for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike XBB.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSF143-wArchon1-KGC-EGFP-ER2

    Plasmid

    #108429

    Purpose

    Codon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    wArchon1-KGC-EGFP-ER2
    

    Expression

    Worm

    Promoter

    flp-18

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5 CMV 3xFG eIF4G1 +MIC

    Plasmid

    #158776

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (with microexon)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. kiCAP-AAV-MyoAAV1E

    Plasmid

    #224439

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 1E, a muscle-tropic AAV capsid in mice.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Mutation

    RGDTMSK insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB

    Plasmid

    #207859

    Purpose

    AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PEmaxDRNaseH-dualU6-Rosa26-twinPE-attB
    

    Use

    AAV

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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